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- Table of Contents
MUC4 antibody (A01652-2) performs well in ELISA, Flow Cytometry, IF, WB and shows reactivity with Human, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of MCF-7 cells using anti-MUC4 antibody (A01652-2).
Overlay histogram showing MCF-7 cells stained with A01652-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-MUC4 Antibody (A01652-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing MCF-7 cells stained with A01652-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-MUC4 Antibody (A01652-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-MUC4 Antibody Picoband®
MUC4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse | Rabbit | ELISA, Flow Cytometry, IF, WB |
Tested Applications
MUC4 antibody was positively detected by WB in:
human Hela whole cell, human U20S whole cell, mouse thymus tissue, mouse lung tissue, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
MUC4 antibody was positively detected by IF in:
mouse stomach tissue
MUC4 antibody was positively detected by FC in:
MCF-7 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Recommend
Mouse
Tested
Recommend
Tested
Recommend
Product Information
Product Name
Anti-MUC4 Antibody Picoband®
View all MUC4 Antibodies
View all MUC4 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MUC4 recombinant protein (Position: Q1250-D1444).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
170 kDa
Calculated Molecular Weight
231.5 kDa
Background of MUC4
Mucin 4, also known as MUC4, is a mucin protein that in humans is encoded by the MUC4 gene. Like other mucins, Mucin 4 is a high-molecular weight glycoprotein. It is mapped to 3q29. In normal functioning, Mucin 4 is known to play anti-adhesive roles in the body, such as in lubricating the reproductive lining. It is found in the respiratory tract, particularly in the trachea and lung and the digestive tract, in the esophagus and colon, as well as in the visual and auditory systems. Mucin 4 serves to protect and lubricate the epithelium, which facilitates transport and traps foreign particles. It is found to be overexpressed during the luteal phase of menstruation. Mucin 4 may also have a role in fetal morphogenic development.
Cite This Product
Anti-MUC4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01652-2)
Protocols
Western Blot protocol for MUC4 antibody
Immunofluorescence (IF) protocol for MUC4 antibody
Flow Cytometry (FC) protocol for MUC4 antibody
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Product Promise
We promise Anti-MUC4 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A01652-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026