Anti-MUC7 Antibody Picoband™

Product Name

Anti-MUC7 Antibody Picoband™

View all MUC7 Antibodies

SKU/Catalog Number

A05210

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MUC7 Antibody Picoband™ catalog # A05210. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MUC7 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05210)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human MUC7.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A05210 is reactive to MUC7 in Human

Applications

A05210 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

46 kDa

Calculated molecular weight

39.159kDa

Background of MUC7

Mucin-7 is a protein that in humans is encoded by the MUC7 gene. This gene encodes a small salivary mucin, which is thought to play a role in facilitating the clearance of bacteria in the oral cavity and to aid in mastication, speech, and swallowing. The central domain of this glycoprotein contains tandem repeats, each composed of 23 amino acids. This antimicrobial protein has antibacterial and antifungal activity. The most common allele contains 6 repeats, and some alleles may be associated with susceptibility to asthma. Alternatively spliced transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot,0.1-0.5μg/ml

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of MUC7 using anti-MUC7 antibody (A05210).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human SW620 whole cell lysates,
Lane 2: human HepG2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUC7 antigen affinity purified polyclonal antibody (Catalog # A05210) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MUC7 at approximately 46KD. The expected band size for MUC7 is at 39KD.

Gene/Protein Information For MUC7 (Source: Uniprot.org, NCBI)

Alternative Names

apo-MG2; DKFZp686J03256; FLJ27047; MG2MGC34772; MUC-7; mucin 7, secreted; mucin-7; Salivary mucin-7; salivary MUC7 MG2 mucin 7, secreted mucin-7|MUC-7|apo-MG2|mucin 7, salivary|salivary mucin-7

For more info on MUC7, check out the MUC7 Infographic

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In this infographic, you will see the following information for MUC7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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