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Product Info Summary
| SKU: | M00815 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Mouse, Rat |
| Host: | Mouse |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-Musk Antibody
SKU/Catalog Number
M00815
Size
80 µl
Description
Boster Bio Anti-Musk Antibody (Catalog # M00815). Tested in IHC-P, WB application(s). This antibody reacts with Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-Musk Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00815)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1429CT456.173.44
Isotype
IgG1,k
Immunogen
This mouse Musk antibody is generated from a mouse immunized with recombinant protein from mouse Musk.
Reactive Species
M00815 is reactive to Musk in Mouse, Rat
Calculated molecular weight
96.7 kDa
Background of Musk
Receptor tyrosine kinase which plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between the motor neuron and the skeletal muscle. Recruitment of AGRIN by LRP4 to the MUSK signaling complex induces phosphorylation and activation of MUSK, the kinase of the complex. The activation of MUSK in myotubes regulates the formation of NMJs through the regulation of different processes including the specific expression of genes in subsynaptic nuclei, the reorganization of the actin cytoskeleton and the clustering of the acetylcholine receptors (AChR) in the postsynaptic membrane. May regulate AChR phosphorylation and clustering through activation of ABL1 and Src family kinases which in turn regulate MUSK. DVL1 and PAK1 that form a ternary complex with MUSK are also important for MUSK-dependent regulation of AChR clustering. May positively regulate Rho family GTPases through FNTA. Mediates the phosphorylation of FNTA which promotes prenylation, recruitment to membranes and activation of RAC1 a regulator of the actin cytoskeleton and of gene expression. Other effectors of the MUSK signaling include DNAJA3 which functions downstream of MUSK. May also play a role within the central nervous system by mediating cholinergic responses, synaptic plasticity and memory formation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00815 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1：2000
IHC-P: 1:25
Validation Images & Assay Conditions
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Western blot analysis of lysates from C2C12, L6 cell line (from left to right), using Musk Antibody. M00815 was diluted at 1:2000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 20μg per lane.
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Immunohistochemical analysis of paraffin-embedded M. skeletal muscle section using Musk Antibody. M00815 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Specific Publications For Anti-Musk Antibody (M00815)
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