Product Info Summary
SKU: | A14099 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-MYBPC2 Antibody
SKU/Catalog Number
A14099
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-MYBPC2 Antibody (Catalog # A14099). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse.
Storage & Handling
MYBPC2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-MYBPC2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14099)
Host
Rabbit
Contents
MYBPC2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
MYBPC2 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human MYBPC2. The immunogen is located within amino acids 1070 - 1120 of MYBPC2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
BX1 antibody is predicted to not cross-react with other DBX protein family members
Reactive Species
A14099 is reactive to MYBPC2 in Human, Mouse
Applications
A14099 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
128.072kDa
Background of MYBPC2
Myosin binding protein C (MYBPC) is a component of the thick filament of striated muscle, with the fast-type isoform designated MYBPC2. Both the fast-type and slow-type MYBPC protein contains seven immunoglobulin C2 motifs and three fibronectin type-III repeats. MYBPC2 is typically required for strong contractions and functions under anaerobic conditions. It is more similar to the cardiac isoform (MYBPC3) than to the slow-type isoform (MYBPC1) in terms of pCa50-indexed force development, length-independent cooperativity and length dependent activation. It has been suggested that in cardiac and fast muscle MYBPC2 contributes to an internal load, possibly by binding to actin via its N-terminal region.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
MYBPC2 antibody can be used for detection of MYBPC2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of MYBPC2 in mouse skeletal muscle tissue lysate with MYBPC2 antibody at (A) 1 and (B) 2 μg/mL.
Click image to see more details
Immunohistochemistry of MYBPC2 in mouse skeletal muscle tissue with MYBPC2 antibody at 5 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For MYBPC2 (Source: Uniprot.org, NCBI)
Gene Name
MYBPC2
Full Name
Myosin-binding protein C, fast-type
Weight
128.072kDa
Superfamily
immunoglobulin superfamily
Alternative Names
C-protein, skeletal muscle fast isoform; fast MyBP-C; fast-type muscle myosin-binding-protein C; MGC163408; MYBPC; MYBPCF; myosin binding protein C, fast type; myosin-binding protein C, fast-type MYBPC2 MYBPC, MYBPCF, fsMyBP-C myosin binding protein C2 myosin-binding protein C, fast-type|fast MyBP-C|fast skeletal-type muscle myosin-binding-protein C|fast-type muscle myosin-binding-protein C|myosin binding protein C, fast type|testicular tissue protein Li 126
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MYBPC2, check out the MYBPC2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MYBPC2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MYBPC2 Antibody (A14099)
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