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Product Info Summary
| SKU: | M00372 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Myeloperoxidase MPO Rabbit Monoclonal Antibody
SKU/Catalog Number
M00372
BM4911 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Myeloperoxidase MPO Rabbit Monoclonal Antibody catalog # M00372. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Myeloperoxidase MPO Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00372)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AOCF-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Myeloperoxidase
Reactive Species
M00372 is reactive to MPO in Human
Observed Molecular Weight
84 kDa
Calculated molecular weight
83.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00372 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
WB: human HL-60 whole cell
Validation Images & Assay Conditions
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Western blot analysis of MPO using anti-MPO antibody (M00372).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MPO antigen affinity purified monoclonal antibody (Catalog # M00372) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MPO at approximately 84 kDa. The expected band size for MPO is at 84 kDa.
Specific Publications For Anti-Myeloperoxidase MPO Rabbit Monoclonal Antibody (M00372)
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4 Customer Q&As for Anti-Myeloperoxidase MPO Rabbit Monoclonal Antibody
Question
My boss were well pleased with the WB result of your anti-Myeloperoxidase Rabbit Monoclonal antibody. However we have observed positive staining in liver lysosome. using this antibody. Is that expected? Could you tell me where is MPO supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
From literature, liver does express MPO. Generally MPO expresses in lysosome. Regarding which tissues have MPO expression, here are a few articles citing expression in various tissues:
Leukemia, Pubmed ID: 2903767
Leukocyte, Pubmed ID: 1334087, 8390465, 2884926
Liver, Pubmed ID: 19159218
Plasma, Pubmed ID: 16335952
Saliva, Pubmed ID: 16740002
Boster Scientific Support
Answered: 2020-02-12
Question
We are currently using anti-Myeloperoxidase Rabbit Monoclonal antibody M00372 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
The anti-Myeloperoxidase Rabbit Monoclonal antibody (M00372) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-02
Question
We have seen staining in human leukemia. Are there any suggestions? Is anti-Myeloperoxidase Rabbit Monoclonal antibody supposed to stain leukemia positively?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
From literature leukemia does express MPO. From Uniprot.org, MPO is expressed in bone marrow cell, leukemia, leukocyte, plasma, saliva, liver, among other tissues. Regarding which tissues have MPO expression, here are a few articles citing expression in various tissues:
Leukemia, Pubmed ID: 2903767
Leukocyte, Pubmed ID: 1334087, 8390465, 2884926
Liver, Pubmed ID: 19159218
Plasma, Pubmed ID: 16335952
Saliva, Pubmed ID: 16740002
Boster Scientific Support
Answered: 2019-06-20
Question
We bought anti-Myeloperoxidase Rabbit Monoclonal antibody for IF on leukocyte a few months ago. I am using human, and We want to use the antibody for WB next. My question regards examining leukocyte as well as bone marrow cell in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
B. Bhatt
Verified customer
Asked: 2016-09-15
Answer
I took a look at the website and datasheets of our anti-Myeloperoxidase Rabbit Monoclonal antibody and I see that M00372 has been validated on human in both IF and WB. Thus M00372 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2016-09-15
