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Product Info Summary
| SKU: | A05031 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-MYLIP Antibody
SKU/Catalog Number
A05031
Size
100μl
Form
Liquid
Description
Boster Bio Anti-MYLIP Antibody catalog # A05031. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MYLIP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05031)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
5G12G2
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human MYLIP. AA range:161-210
Reactive Species
A05031 is reactive to MYLIP in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
49.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05031 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MYLIP Antibody. The picture on the right is blocked with the synthesized peptide.
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Western Blot analysis of Jurkat cells using MYLIP Polyclonal Antibody
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Western blot analysis of lysates from HeLa and Jurkat cells, using MYLIP Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-MYLIP Antibody (A05031)
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-MYLIP Antibody
Question
Is this A05031 anti-MYLIP antibody reactive to the isotypes of MYLIP?
W. Thomas
Verified customer
Asked: 2020-01-27
Answer
The immunogen of A05031 anti-MYLIP antibody is Synthesized peptide derived from the Internal region of human MYLIP. at AA rangle: 130-210. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-27
Question
Does anti-MYLIP antibody A05031 work on pig WB with placenta?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
Our lab technicians have not tested anti-MYLIP antibody A05031 on pig. You can run a BLAST between pig and the immunogen sequence of anti-MYLIP antibody A05031 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig placenta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-09
Question
We are currently using anti-MYLIP antibody A05031 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
The anti-MYLIP antibody (A05031) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-08
Question
Will anti-MYLIP antibody A05031 work for WB with brain?
G. Huang
Verified customer
Asked: 2019-11-06
Answer
According to the expression profile of brain, MYLIP is highly expressed in brain. So, it is likely that anti-MYLIP antibody A05031 will work for WB with brain.
Boster Scientific Support
Answered: 2019-11-06
Question
Do you have a BSA free version of anti-MYLIP antibody A05031 available?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MYLIP antibody A05031 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-28
Question
you antibody to test anti-MYLIP antibody A05031 on human brain for research purposes, then I may be interested in using anti-MYLIP antibody A05031 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-10-22
Answer
The products we sell, including anti-MYLIP antibody A05031, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-10-22
Question
Is a blocking peptide available for product anti-MYLIP antibody (A05031)?
Verified Customer
Verified customer
Asked: 2018-02-19
Answer
We do provide the blocking peptide for product anti-MYLIP antibody (A05031). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-02-19
Question
I was wanting to use your anti-MYLIP antibody for WB for human brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human brain identification?
Verified Customer
Verified customer
Asked: 2017-09-12
Answer
It shows on the product datasheet, A05031 anti-MYLIP antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-12
Question
Does A05031 anti-MYLIP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-09-08
Answer
As indicated on the product datasheet, A05031 anti-MYLIP antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-09-08
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-MYLIP antibody A05031. Let me know if you need anything else.
L. Zhao
Verified customer
Asked: 2015-10-12
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-10-12
Question
I see that the anti-MYLIP antibody A05031 works with WB, what is the protocol used to produce the result images on the product page?
J. Jones
Verified customer
Asked: 2014-10-08
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2014-10-08
Question
Can you help my question with product A05031, anti-MYLIP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
V. Jackson
Verified customer
Asked: 2014-09-22
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05031 anti-MYLIP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-09-22
Question
I have attached the WB image, lot number and protocol we used for brain using anti-MYLIP antibody A05031. Please let me know if you require anything else.
A. Moore
Verified customer
Asked: 2014-04-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-04-02
