Anti-MYLIP Antibody

MYLIP antibody

Boster Bio Anti-MYLIP Antibody catalog # A05031. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A05031
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-MYLIP Antibody

View all MYLIP Antibodies

SKU/Catalog Number

A05031

Size

100μl

Form

Liquid

Description

Boster Bio Anti-MYLIP Antibody catalog # A05031. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MYLIP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05031)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Monoclonal

Clone Number

5G12G2

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human MYLIP. at AA rangle: 130-210

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05031 is reactive to MYLIP in Human, Mouse, Rat

Applications

A05031 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

49.91kDa

Background of MYLIP

May play an important role in cell fate determination during lung development and in spermatogenesis.

Li C.-S., Chae S.-C., Lee J.-H., Zhang Q., Chung H.-T.J. Hum. Genet. 51:292-297(2006)

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For MYLIP (Source: Uniprot.org, NCBI)

Gene Name

MYLIP

Full Name

E3 ubiquitin-protein ligase MYLIP

Weight

49.91kDa

Alternative Names

band 4.1 superfamily member BZF1; BZF1; cellular modulator of immune recognition (c-MIR); EC 6.3.2.-; Idol; IDOLmyosin regulatory light chain-interacting protein; Inducible degrader of the LDL-receptor; MIRE3 ubiquitin-protein ligase MYLIP; myosin regulatory light chain interacting proteinE3 ubiquitin ligase-inducible degrader of the low density lipoprotein receptor MYLIP IDOL, MIR myosin regulatory light chain interacting protein E3 ubiquitin-protein ligase MYLIP|E3 ubiquitin ligase-inducible degrader of the low density lipoprotein receptor|RING-type E3 ubiquitin transferase MYLIP|band 4.1 superfamily member BZF1|cellular modulator of immune recognition (c-MIR)|inducible degrader of the LDL-receptor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MYLIP, check out the MYLIP Infographic

MYLIP infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MYLIP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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13 Customer Q&As for Anti-MYLIP Antibody

Question

Is this A05031 anti-MYLIP antibody reactive to the isotypes of MYLIP?

W. Thomas

Verified customer

Asked: 2020-01-27

Answer

The immunogen of A05031 anti-MYLIP antibody is Synthesized peptide derived from the Internal region of human MYLIP. at AA rangle: 130-210. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-01-27

Question

Does anti-MYLIP antibody A05031 work on pig WB with placenta?

Verified Customer

Verified customer

Asked: 2019-12-09

Answer

Our lab technicians have not tested anti-MYLIP antibody A05031 on pig. You can run a BLAST between pig and the immunogen sequence of anti-MYLIP antibody A05031 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig placenta in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-12-09

Question

We are currently using anti-MYLIP antibody A05031 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2019-11-08

Answer

The anti-MYLIP antibody (A05031) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-08

Question

Will anti-MYLIP antibody A05031 work for WB with brain?

G. Huang

Verified customer

Asked: 2019-11-06

Answer

According to the expression profile of brain, MYLIP is highly expressed in brain. So, it is likely that anti-MYLIP antibody A05031 will work for WB with brain.

Boster Scientific Support

Answered: 2019-11-06

Question

Do you have a BSA free version of anti-MYLIP antibody A05031 available?

Verified Customer

Verified customer

Asked: 2019-06-28

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MYLIP antibody A05031 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-06-28

Question

you antibody to test anti-MYLIP antibody A05031 on human brain for research purposes, then I may be interested in using anti-MYLIP antibody A05031 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-10-22

Answer

The products we sell, including anti-MYLIP antibody A05031, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-10-22

Question

Is a blocking peptide available for product anti-MYLIP antibody (A05031)?

Verified Customer

Verified customer

Asked: 2018-02-19

Answer

We do provide the blocking peptide for product anti-MYLIP antibody (A05031). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-02-19

Question

I was wanting to use your anti-MYLIP antibody for WB for human brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human brain identification?

Verified Customer

Verified customer

Asked: 2017-09-12

Answer

It shows on the product datasheet, A05031 anti-MYLIP antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-09-12

Question

Does A05031 anti-MYLIP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-09-08

Answer

As indicated on the product datasheet, A05031 anti-MYLIP antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-09-08

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-MYLIP antibody A05031. Let me know if you need anything else.

L. Zhao

Verified customer

Asked: 2015-10-12

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-10-12

Question

I see that the anti-MYLIP antibody A05031 works with WB, what is the protocol used to produce the result images on the product page?

J. Jones

Verified customer

Asked: 2014-10-08

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2014-10-08

Question

Can you help my question with product A05031, anti-MYLIP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

V. Jackson

Verified customer

Asked: 2014-09-22

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05031 anti-MYLIP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2014-09-22

Question

I have attached the WB image, lot number and protocol we used for brain using anti-MYLIP antibody A05031. Please let me know if you require anything else.

A. Moore

Verified customer

Asked: 2014-04-02

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-04-02

Order DetailsPrice
A05031

100uL

$399

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