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Product Info Summary
| SKU: | A03238-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Myogenin/MYOG Antibody Picoband®
SKU/Catalog Number
A03238-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-Myogenin/MYOG Antibody Picoband® catalog # A03238-1. Tested in ELISA, WB applications. This antibody reacts with Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Myogenin/MYOG Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03238-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human Myogenin/MYOG recombinant protein (Position: M1-N224).
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A03238-1 is reactive to MYOG in Monkey, Mouse, Rat
Observed Molecular Weight
40 kDa
Calculated molecular weight
25.0 kDa
Background of MYOG
Myogenin, is a transcriptional activator encoded by the MYOG gene. Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix (HLH) proteins. It is essential for the development of functional skeletal muscle.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03238-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Monkey, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: monkey skeletal muscle tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue
Validation Images & Assay Conditions
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Western blot analysis of Myogenin/MYOG using anti-Myogenin/MYOG antibody (A03238-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: monkey skeletal muscle tissue lysates,
Lane 2: rat skeletal muscle tissue lysates,
Lane 3: mouse skeletal muscle tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Myogenin/MYOG antigen affinity purified polyclonal antibody (Catalog # A03238-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Myogenin/MYOG at approximately 40 kDa. The expected band size for Myogenin/MYOG is at 25 kDa.
Specific Publications For Anti-Myogenin/MYOG Antibody Picoband® (A03238-1)
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