Anti-Myomesin-2 MYOM2 Antibody

Myomesin 2 antibody

Boster Bio Anti-Myomesin-2 MYOM2 Antibody catalog # A12094. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse.

Product Info Summary

SKU: A12094
Size: 100μl
Reactive Species: Human, Monkey, Mouse
Host: Rabbit
Application: WB

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Product Name

Anti-Myomesin-2 MYOM2 Antibody

View all Myomesin 2 Antibodies

SKU/Catalog Number

A12094

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Myomesin-2 MYOM2 Antibody catalog # A12094. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Myomesin-2 MYOM2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12094)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

F7E4

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human Myomesin-2. at AA rangle: 590-670

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A12094 is reactive to MYOM2 in Human, Monkey, Mouse

Applications

A12094 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

164.896kDa

Background of Myomesin 2

COBRA1 (cofactor of BRCA1) also known as NELF-B, is an essential component of the negative elongation factor (NELF) complex. COBRA1 has been shown to directly bind estrogen receptor alpha (ERα) and repress ERα-mediated transcription (Aiyar SE et al., 2004). COBRA1 has also been demonstrated to interact with c-Jun and c-Fos suggesting a role in modulation of the AP-1 pathway and therefore may be important in cell proliferation, differentiation, apoptosis and oncogenesis (Zhong H et al., 2004)

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For MYOM2 (Source: Uniprot.org, NCBI)

Gene Name

MYOM2

Full Name

Myomesin-2

Weight

164.896kDa

Alternative Names

165 kDa connectin-associated protein; 165 kDa titin-associated protein; M-band protein; M-protein; myomesin (M-protein) 2 (165kD); myomesin (M-protein) 2, 165kDa; Myomesin family member 2; myomesin-2; titin-associated protein, 165 kD; TTNAP MYOM2 TTNAP myomesin 2 myomesin-2|165 kDa connectin-associated protein|165 kDa titin-associated protein|M-band protein|M-protein|myomesin (M-protein) 2, 165kDa|myomesin family member 2|titin-associated protein, 165 kD

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MYOM2, check out the MYOM2 Infographic

MYOM2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MYOM2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-Myomesin-2 MYOM2 Antibody

Question

Does A12094 anti-Myomesin-2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-12-05

Answer

You can see on the product datasheet, A12094 anti-Myomesin-2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-12-05

Question

We are currently using anti-Myomesin-2 antibody A12094 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, monkey, mouse. Is it likely that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2018-04-17

Answer

The anti-Myomesin-2 antibody (A12094) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-04-17

Question

Is this A12094 anti-Myomesin-2 antibody reactive to the isotypes of MYOM2?

F. Krishna

Verified customer

Asked: 2017-11-08

Answer

The immunogen of A12094 anti-Myomesin-2 antibody is Synthesized peptide derived from the Internal region of human Myomesin-2. at AA rangle: 590-670. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-11-08

Question

I see that the anti-Myomesin-2 antibody A12094 works with WB, what is the protocol used to produce the result images on the product page?

P. Zhang

Verified customer

Asked: 2014-03-04

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2014-03-04

Question

Would anti-Myomesin-2 antibody A12094 work on monkey WB with apex of heart?

S. Krishna

Verified customer

Asked: 2013-07-25

Answer

Our lab technicians have not tested anti-Myomesin-2 antibody A12094 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Myomesin-2 antibody A12094 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey apex of heart in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-07-25

Question

We are interested in to test anti-Myomesin-2 antibody A12094 on monkey apex of heart for research purposes, then I may be interested in using anti-Myomesin-2 antibody A12094 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

O. Zhang

Verified customer

Asked: 2013-03-25

Answer

The products we sell, including anti-Myomesin-2 antibody A12094, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2013-03-25

Order DetailsPrice
A12094

100uL

$399

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