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Product Info Summary
| SKU: | A12094 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Monkey, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Myomesin-2 MYOM2 Antibody
SKU/Catalog Number
A12094
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Myomesin-2 MYOM2 Antibody catalog # A12094. Tested in WB, ELISA, IHC applications. This antibody reacts with Human, Mouse, Monkey.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Myomesin-2 MYOM2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12094)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human MYOM2. AA range:612-661
Reactive Species
A12094 is reactive to MYOM2 in Human, Monkey, Mouse
Calculated molecular weight
164.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12094 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
ELISA 1:2000-20000
Validation Images & Assay Conditions
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Western blot analysis of lysates from COS7 and HT-29 cells, using MYOM2 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-Myomesin-2 MYOM2 Antibody (A12094)
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Customer Q&As
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6 Customer Q&As for Anti-Myomesin-2 MYOM2 Antibody
Question
Does A12094 anti-Myomesin-2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
You can see on the product datasheet, A12094 anti-Myomesin-2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-05
Question
We are currently using anti-Myomesin-2 antibody A12094 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, monkey, mouse. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-04-17
Answer
The anti-Myomesin-2 antibody (A12094) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-04-17
Question
Is this A12094 anti-Myomesin-2 antibody reactive to the isotypes of MYOM2?
F. Krishna
Verified customer
Asked: 2017-11-08
Answer
The immunogen of A12094 anti-Myomesin-2 antibody is Synthesized peptide derived from the Internal region of human Myomesin-2. at AA rangle: 590-670. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-11-08
Question
I see that the anti-Myomesin-2 antibody A12094 works with WB, what is the protocol used to produce the result images on the product page?
P. Zhang
Verified customer
Asked: 2014-03-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2014-03-04
Question
Would anti-Myomesin-2 antibody A12094 work on monkey WB with apex of heart?
S. Krishna
Verified customer
Asked: 2013-07-25
Answer
Our lab technicians have not tested anti-Myomesin-2 antibody A12094 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Myomesin-2 antibody A12094 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey apex of heart in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-07-25
Question
We are interested in to test anti-Myomesin-2 antibody A12094 on monkey apex of heart for research purposes, then I may be interested in using anti-Myomesin-2 antibody A12094 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
O. Zhang
Verified customer
Asked: 2013-03-25
Answer
The products we sell, including anti-Myomesin-2 antibody A12094, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-03-25
