Anti-Myosin 1G MYO1G Antibody

Product Name

Anti-Myosin 1G MYO1G Antibody

View all MYO1G Antibodies

SKU/Catalog Number

A08448

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Myosin 1G MYO1G Antibody (Catalog # A08448). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Myosin 1G MYO1G Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08448)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal region of human Myosin 1G protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A08448 is reactive to MYO1G in Human

Applications

A08448 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

116.442kDa

Background of MYO1G

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. In general, myosins are protein complexes consisting of one or more myosin heavy chains, associated light chains and other proteins. Myosins function as molecular motors and use the energy of ATP hydrolysis to move actin filaments or to move vesicles or other cargo on fixed actin filaments. Myosins have magnesium-ATPase activity and bind actin. Myosins can be divided into classes that are distinguished based on sequence features of the motor, or head domain, but also have distinct tail regions that are believed to bind specific cargoes. Unconventional myosins exist. Myosin 1G is an unconventional myosin that is restricted to hematopoietic cells. Unconventional myosins are also critical for motility in amoeba and a mammalian paralog (Myo1C) is critical as a glucose transporter that recycles glucose in response to insulin.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:40,000
IP: 1:100
WB: 1:500 - 1:3,000

Validation Images & Assay Conditions

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Western blot analysis of Myosin 1G expression in Jurkat whole cell lysates (lane 1), peripheral blood T cells lysates (lane 2), human spleen extract (lane 3), 300.19 (lane 4) and 293 cells, appear negative for Myosin 1G (lane 5). Myosin 1G at 70KD was detected using rabbit anti-FBXL4 Antigen Affinity purified polyclonal antibody (Catalog # ) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

Gene/Protein Information For MYO1G (Source: Uniprot.org, NCBI)

Alternative Names

Unconventional myosin-Ig MYO1G HA2, HLA-HA2, MHAG myosin IG unconventional myosin-Ig|minor histocompatibility antigen HA-2|myosin-Ig

For more info on MYO1G, check out the MYO1G Infographic

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In this infographic, you will see the following information for MYO1G: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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