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Product Info Summary
| SKU: | M01697 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Myosin light chain kinase MYLK Rabbit Monoclonal Antibody
SKU/Catalog Number
M01697
BM4290 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Myosin light chain kinase MYLK Rabbit Monoclonal Antibody catalog # M01697. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Myosin light chain kinase MYLK Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01697)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
G-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Myosin light chain kinase
Reactive Species
M01697 is reactive to MYLK in Human, Mouse, Rat
Observed Molecular Weight
130, 210 kDa
Calculated molecular weight
210.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01697 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
Positive Control
WB: HUVEC cell
IHC: human colon tissue
Validation Images & Assay Conditions
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Western blot analysis of myosin light chain kinase expression in HUVEC cell lysate.
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The co-expression of EspF and ANXA6 protein in Caco-2 cells activates the MLCK-MLC pathway. The WB strips of MLCK-MLC pathway proteins in different plasmid-transfection groups, as indicated, are shown. Increased expression of MLCK, phosphorylated expression of MLC and PKCα, and decreased expression of Calmodulin in the EspF-ANXA6 transfected group, suggesting that EspF-ANXA6 activates the MLCK-MLC pathway, which may perturb TJ function. NC, non-transfected cells; EY, cells transfected with pEYFP; EYEA, cells transfected with pEYFP-EspF-T2A-ANXA6; EE, cells transfected with pEGFP-EspF. *Statistically significant difference. * p < 0.05. ** p < 0.01.
Index in PubMed under a CC BY license. PMID: 33425920
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Immunohistochemical analysis of paraffin-embedded human colon, using Myosin light chain kinase Antibody.
Specific Publications For Anti-Myosin light chain kinase MYLK Rabbit Monoclonal Antibody (M01697)
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4 Customer Q&As for Anti-Myosin light chain kinase MYLK Rabbit Monoclonal Antibody
Question
Does anti-Myosin light chain kinase Rabbit Monoclonal antibody M01697 work on dog WB with umbilical vein endothelial cell?
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
Our lab technicians have not tested anti-Myosin light chain kinase Rabbit Monoclonal antibody M01697 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Myosin light chain kinase Rabbit Monoclonal antibody M01697 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog umbilical vein endothelial cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-18
Question
See below the WB image, lot number and protocol we used for umbilical vein using anti-Myosin light chain kinase Rabbit Monoclonal antibody M01697. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-09
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for umbilical vein using anti-Myosin light chain kinase Rabbit Monoclonal antibody M01697. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-06
Question
I would like to test anti-Myosin light chain kinase Rabbit Monoclonal antibody M01697 on human umbilical vein for research purposes, then I may be interested in using anti-Myosin light chain kinase Rabbit Monoclonal antibody M01697 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-07-12
Answer
The products we sell, including anti-Myosin light chain kinase Rabbit Monoclonal antibody M01697, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-07-12
