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- Table of Contents
MYLK antibody (M01697) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Immunohistochemical analysis of paraffin-embedded human colon, using Myosin light chain kinase Antibody.
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Anti-Myosin light chain kinase MYLK Rabbit Monoclonal Antibody
MYLK antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
MYLK antibody was positively detected by WB in:
HUVEC cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
MYLK antibody was positively detected by IHC in:
human colon tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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IHC
ICC
IF
FC
Human
Tested
Recommend
Recommend
Recommend
Mouse
Recommend
Recommend
Recommend
Rat
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-Myosin light chain kinase MYLK Rabbit Monoclonal Antibody
View all MYLK Antibodies
View all MYLK Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
G-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Myosin light chain kinase
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
130, 210 kDa
Calculated Molecular Weight
210.7 kDa
Cite This Product
Anti-Myosin light chain kinase MYLK Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01697)
Protocols
Western Blot protocol for MYLK antibody
Immunohistochemistry (IHC) protocol for MYLK antibody
Publications
Application:
Species:
Enterohemorrhagic Escherichia coli Effector Protein EspF Interacts With Host Protein ANXA6 and Triggers Myosin Light Chain Kinase (MLCK)-Dependent Tight Junction Dysregulation
Published: 2020-12-23
Journal: Frontiers in Cell and Developmental Biology
Application: WB
Impact Factor: 5.19
Species: Human
PMID: 33425920
Review
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Product Promise
We promise Anti-Myosin light chain kinase MYLK Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
M01697
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026