|Reactivity||Human, Mouse, Rabbit, Rat|
|Ig Isotype||Mouse IgG1|
|Product Name||Anti-Myosin(Skeletal, Slow) Antibody (Monoclonal, NOQ7.5.4D)|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for Myosin (Skeletal, Slow), myosin, heavy chain 7, cardiac muscle, beta (MYH7) detection. Tested with WB, IHC-P in Human;mouse;rabbit;rat. No cross reactivity with other proteins.|
|Cite This Product||Anti-Myosin(Skeletal, Slow) Antibody (Monoclonal, NOQ7.5.4D) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1064)|
|Contents/Buffer||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Immunogen||Human skeletal muscle myosin purified from myofibrils.|
|Reactivity||Human, Mouse, Rabbit, Rat|
Assay Dilutions Overview
Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rabbit, rat, By Heat
Western blot, 0.5-2μg/ml, Human, mouse, rabbit, rat
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
Images And Assay Conditions
Figure 1. Western blot analysis of Myosin(Skeletal, Slow) using anti- Myosin(Skeletal, Slow) antibody (MA1064).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse skeletal muscle tissue lysates,
Lane 2: rat skeletal muscle tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- Myosin(Skeletal, Slow) antigen affinity purified monoclonal antibody (Catalog # MA1064) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Myosin(Skeletal, Slow) at approximately 200-220KD. The expected band size for Myosin(Skeletal, Slow) is at 220KD.
Protein Target Info (Source: Uniprot.org)
|Tissue Specificity||MYH7: Both wild type and variant Gln-403 aredetected in skeletal muscle (at protein level).|
|Alternative Names||Myosin-7;Myosin heavy chain 7;Myosin heavy chain slow isoform;MyHC-slow;Myosin heavy chain, cardiac muscle beta isoform;MyHC-beta;Myh7;|
|Subcellular Localization||Cytoplasm, myofibril. Thick filaments of the myofibrils.|
|Molecular Weight||223083 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Muscle contraction.|
|Research Areas||Cytoskeleton, Cytoskeleton / Ecm, Motor Proteins, Myosin, Signal Transduction
*You can search these to find other products in these research areas.
|Background||Myosin is composed of 2 heavy chains of about 200,000 daltons each and 4 light chains of about 20,000 daltons each. Skeletal Myosin(slow),, also known as light chain 3(MYL3), mapped to 3p. Fodor et al.(1989) found that the MYL3 gene has 7 exons, the last of which is completely untranslated 3-prime sequence.|
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Order Product (MA1064)
Promotion:Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.Download Free PDFs Now
Guaranteed product quality
We promise all of our products perform as described in datasheets.