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Product Info Summary
|Reactive Species:||Human, Mouse, Rabbit, Rat|
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Anti-Myosin(Skeletal, Slow) Myh7 Antibody (Monoclonal, NOQ7.5.4D)
Boster Bio Anti-Myosin (Skeletal, Slow) Myh7 Antibody (Monoclonal, NOQ7.5.4D) catalog # MA1064. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rabbit, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Myosin(Skeletal, Slow) Myh7 Antibody (Monoclonal, NOQ7.5.4D) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1064)
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Human skeletal muscle myosin purified from myofibrils.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins
MA1064 is reactive to Myh7 in Human, Mouse, Rabbit, Rat
MA1064 is guaranteed for IHC, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rabbit, rat, By Heat
Western blot, 0.5-2μg/ml, Human, mouse, rabbit, rat
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Myosin(Skeletal, Slow) using anti-Myosin(Skeletal, Slow) antibody (MA1064).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse skeletal muscle tissue lysates,
Lane 2: rat skeletal muscle tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Myosin(Skeletal, Slow) antigen affinity purified monoclonal antibody (Catalog # MA1064) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Myosin(Skeletal, Slow) at approximately 200-220KD. The expected band size for Myosin(Skeletal, Slow) is at 220KD.
Gene/Protein Information For Myh7 (Source: Uniprot.Org, NCBI)
TRAFAC class myosin-kinesin ATPase superfamily
cardiac muscle beta isoform; CMD1S; CMD1SMGC138376; CMH1; DKFZp451F047; MGC138378; MPD1; MYH7; MYHCB; MyHC-beta; myhc-slow; myopathy, distal 1; myosin heavy chain (AA 1-96); myosin, heavy chain 7, cardiac muscle, beta; myosin, heavy polypeptide 7, cardiac muscle, beta; Myosin-7; rhabdomyosarcoma antigen MU-RMS-40.7A; SPMD; SPMM MYH7 CMD1S, CMH1, MPD1, MYHCB, SPMD, SPMM myosin heavy chain 7 myosin-7|cardiac muscle myosin heavy chain 7 beta|myHC-beta|myhc-slow|myopathy, distal 1|myosin 7|myosin heavy chain beta-subunit|myosin, heavy chain 7, cardiac muscle, beta|myosin, heavy polypeptide 7, cardiac muscle, beta|rhabdomyosarcoma antigen MU-RMS-40.7A*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Myh7, check out the Myh7 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Myh7: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Anti-Myosin(Skeletal, Slow) Myh7 Antibody (Monoclonal, NOQ7.5.4D) (MA1064)
MA1064 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Yan X,Niu Q,Gao X,Shen S,He N,Wang H,Fang R,Gao Y,Chang H.2021.Differential protein metabolism and regeneration in gastrocnemius muscles in high-fat diet fed mice and pre-hibernation Daurian ground squirrels: a comparison between pathological and healthy
Nie Yw, Ding Xb, Ge Xg, Fan Hl, Liu Zw, Guo H. Cell Biol Int. 2013 Sep;37(9):972-6. Doi: 10.1002/Cbin.10124. Epub 2013 May 23. Enhanced Expression Of Myf5 And Myod1 In Fibroblast Cells Via The Forced Expression Of Bos Taurus Myf5.
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