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SKU:MA1064
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Monoclonal
Clone Number:NOQ7.5.4D
Application:IHC, WB
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Overview

Product Name Anti-Myosin(Skeletal, Slow) Antibody (Monoclonal, NOQ7.5.4D)
SKU/Catalog Number MA1064
Description Mouse IgG monoclonal antibody for Myosin (Skeletal, Slow), myosin, heavy chain 7, cardiac muscle, beta (MYH7) detection. Tested with WB, IHC-P in Human;mouse;rabbit;rat. No cross reactivity with other proteins.
Cite This Product Anti-Myosin(Skeletal, Slow) Antibody (Monoclonal, NOQ7.5.4D) NOQ7.5.4D (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1064)
Replacement Item This antibody may replace the following items: sc-32733|sc-168678|sc-53090|sc-53089|sc-71575 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Human, Mouse, Rabbit, Rat
Application IHC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Human skeletal muscle myosin purified from myofibrils.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number NOQ7.5.4D
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Myh7
Protein Name Myosin-7
Molecular Weight 223083 MW
Protein Function Muscle contraction.
Tissue Specificity MYH7: Both wild type and variant Gln-403 aredetected in skeletal muscle (at protein level).
Sequence Similarities Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.
Subcellular Localization Cytoplasm, myofibril. Thick filaments of the myofibrils.
Uniprot ID P02564
Alternative Names Myosin-7;Myosin heavy chain 7;Myosin heavy chain slow isoform;MyHC-slow;Myosin heavy chain, cardiac muscle beta isoform;MyHC-beta;Myh7;
Research Areas |signal transduction|cytoskeleton / ecm|cytoskeleton|motor proteins|myosin|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Myosin-7

Myosin is composed of 2 heavy chains of about 200,000 daltons each and 4 light chains of about 20,000 daltons each. Skeletal Myosin(slow),, also known as light chain 3(MYL3), mapped to 3p. Fodor et al.(1989) found that the MYL3 gene has 7 exons, the last of which is completely untranslated 3-prime sequence.

Anti-Myosin(Skeletal, Slow) Antibody (Monoclonal, NOQ7.5.4D) Images

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Anti-Myosin(Skeletal, Slow) Antibody (Monoclonal, NOQ7.5.4D)
Anti-Myosin(Skeletal, Slow) antibody (monoclonal), MA1064, Western blotting
WB: Rat Skeletal Muscle Tissue Lysate
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Publications

Nie Yw, Ding Xb, Ge Xg, Fan Hl, Liu Zw, Guo H. Cell Biol Int. 2013 Sep;37(9):972-6. Doi: 10.1002/Cbin.10124. Epub 2013 May 23. Enhanced Expression Of Myf5 And Myod1 In Fibroblast Cells Via The Forced Expression Of Bos Taurus Myf5.

Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: One other very common name is myosin light chain 3 antibody