SKU MA1064
Size 100μg/vial
Reactivity Human, Mouse, Rabbit, Rat
Clonality Monoclonal
Clone Number NOQ7.5.4D
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, WB

Overview

Product Name Anti-Myosin(Skeletal, Slow) Antibody (Monoclonal, NOQ7.5.4D)
SKU/Catalog Number MA1064
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Myosin (Skeletal, Slow), myosin, heavy chain 7, cardiac muscle, beta (MYH7) detection. Tested with WB, IHC-P in Human;mouse;rabbit;rat. No cross reactivity with other proteins.
Cite This Product Anti-Myosin(Skeletal, Slow) Antibody (Monoclonal, NOQ7.5.4D) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1064)
Host Mouse
Immunogen Human skeletal muscle myosin purified from myofibrils.
Reactivity Human, Mouse, Rabbit, Rat

Assay Details

Assay Dilutions Overview

Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rabbit, rat, By Heat
Western blot, 0.5-2μg/ml, Human, mouse, rabbit, rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).

Images And Assay Conditions

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Figure 1. Western blot analysis of Myosin(Skeletal, Slow) using anti- Myosin(Skeletal, Slow) antibody (MA1064).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse skeletal muscle tissue lysates,
Lane 2: rat skeletal muscle tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- Myosin(Skeletal, Slow) antigen affinity purified monoclonal antibody (Catalog # MA1064) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Myosin(Skeletal, Slow) at approximately 200-220KD. The expected band size for Myosin(Skeletal, Slow) is at 220KD.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P02564
Gene Name Myh7
Protein Name Myosin-7
Tissue Specificity MYH7: Both wild type and variant Gln-403 aredetected in skeletal muscle (at protein level).
Alternative Names Myosin-7;Myosin heavy chain 7;Myosin heavy chain slow isoform;MyHC-slow;Myosin heavy chain, cardiac muscle beta isoform;MyHC-beta;Myh7;
Subcellular Localization Cytoplasm, myofibril. Thick filaments of the myofibrils.
Molecular Weight 223083 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Muscle contraction.
Background Myosin is composed of 2 heavy chains of about 200,000 daltons each and 4 light chains of about 20,000 daltons each. Skeletal Myosin(slow),, also known as light chain 3(MYL3), mapped to 3p. Fodor et al.(1989) found that the MYL3 gene has 7 exons, the last of which is completely untranslated 3-prime sequence.

Order Product (MA1064)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280
Ships in 5-7 business days.

Troubleshooting

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Publications

Nie Yw, Ding Xb, Ge Xg, Fan Hl, Liu Zw, Guo H. Cell Biol Int. 2013 Sep;37(9):972-6. Doi: 10.1002/Cbin.10124. Epub 2013 May 23. Enhanced Expression Of Myf5 And Myod1 In Fibroblast Cells Via The Forced Expression Of Bos Taurus Myf5.

Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: One other very common name is myosin light chain 3 antibody