|Reactivity||Chicken, Human, Mouse, Rabbit, Rat|
|Ig Isotype||Mouse IgG1|
|Product Name||Anti-Cadherin-2/N-Cadherin Monoclonal Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for N-Cadherin, cadherin 2, type 1, N-cadherin (neuronal) (CDH2 ) detection. Tested with WB, IHC-F in Human;mouse;rat;rabbit;chicken. No cross reactivity with other proteins.|
|Cite This Product||Anti-Cadherin-2/N-Cadherin Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1067)|
|Contents/Buffer||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Immunogen||Affinity purified chicken heart A-CAM.|
|Reactivity||Chicken, Human, Mouse, Rabbit, Rat|
Assay Dilutions Overview
Immunohistochemistry(Frozen Section), 4μg/ml, Human, mouse, rat, rabbit, chicken, -
Western blot, 2μg/ml, Human, mouse, rat, rabbit, chicken
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F).
Images And Assay Conditions
Figure 1. Western blot analysis of Cadherin-2/N-Cadherin using anti- Cadherin-2/N-Cadherin antibody (MA1067).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human PANC-1 whole cell lysates,
Lane 5: human A549 whole cell lysates,
Lane 6: mouse NIH3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- Cadherin-2/N-Cadherin antigen affinity purified monoclonal antibody (Catalog # MA1067) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Cadherin-2/N-Cadherin at approximately 130KD. The expected band size for Cadherin-2/N-Cadherin is at 100KD.
Protein Target Info (Source: Uniprot.org)
|Tissue Specificity||In testis, expressed in Sertoli and germ cells. .|
|Alternative Names||Cadherin-2;Neural cadherin;N-cadherin;CD325;Cdh2;|
|Subcellular Localization||Cell membrane ; Single-pass type I membrane protein .|
|Molecular Weight||99686 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density (By similarity). .|
|Research Areas||Cadherins, Cell Adhesion, Cytoskeleton / Ecm, Signal Transduction
*You can search these to find other products in these research areas.
|Background||N-cadherin(NCAD) is a member of the cadherin cell-cell adhesion receptor family that includes P-, E-, and R-cadherin and liver cell adhesion molecule(L-CAM). N-Cadherin,, also known as Cadherin-2, encodes a 907-amino acid protein that includes a 159-amino acid signal sequence. Human and mouse nucleotide sequences are 96% identical. Mouse Ncad gene consists of 16 exons dispersed over more than 200 kb of genomic DNA. Human N-cadherin gene contains 16 exons and its sequence is highly similar to both the mouse NCAD gene(including the large first and second introns) and other cadherin genes. N-cadherin is mapped to 18q11.2. Cadherin regulates dendritic spine morphogenesis.|
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