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Product Info Summary
|Reactive Species:||Chicken, Human, Mouse, Rabbit, Rat|
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Anti-N-Cadherin-2 CDH2 CD325-Monoclonal Antibody
Boster Bio Anti-N-Cadherin-2 CDH2 CD325-Monoclonal Antibody catalog # MA1067. Tested in IHC, WB applications. This antibody reacts with Chicken, Human, Mouse, Rabbit, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-N-Cadherin-2 CDH2 CD325-Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1067)
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Affinity purified chicken heart A-CAM.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins
MA1067 is reactive to Cdh2 in Chicken, Human, Mouse, Rabbit, Rat
MA1067 is guaranteed for IHC, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Immunohistochemistry (Frozen Section), 4μg/ml, Human, mouse, rat, rabbit, chicken, -
Western blot, 2μg/ml, Human, mouse, rat, rabbit, chicken
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Cadherin-2/N-Cadherin using anti-Cadherin-2/N-Cadherin antibody (MA1067).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human PANC-1 whole cell lysates,
Lane 5: human A549 whole cell lysates,
Lane 6: mouse NIH3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cadherin-2/N-Cadherin antigen affinity purified monoclonal antibody (Catalog # MA1067) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Cadherin-2/N-Cadherin at approximately 130KD. The expected band size for Cadherin-2/N-Cadherin is at 100KD.
Gene/Protein Information For Cdh2 (Source: Uniprot.Org, NCBI)
cadherin 2, N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; CD325 antigen; CD325; CDH2; CDHNcalcium-dependent adhesion protein, neuronal; CDw325; NCadherin; N-Cadherin; NCADN-cadherin 1; Neural cadherin; neural-cadherin Cdh2|N-cadherin|cadherin 2|cadherin-2|cadherin 2, type 1, N-cadherin (neuronal)|neural cadherin*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Cdh2, check out the Cdh2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Cdh2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
MA1067 has been cited in 21 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Piao HY,Guo S,Wang Y,Zhang J.Exosome-transmitted lncRNA PCGEM1 promotes invasive and metastasis in gastric cancer by maintaining the stability of SNAI1.Clin Transl Oncol.2020 Jun 9.doi:10.1007/s12094-020-02412-9.Epub ahead of print.PMID:32519176.
MA1067 usage in article: APP:WB, SAMPLE:AGS CELL AND MKN45 CELL, DILUTION:1:200
Shi R,Liu L,Wang F,He Y,Niu Y,Wang C,Zhang X,Zhang X,Zhang H,Chen M,Wang Y.Downregulation of cytokeratin 18 induces cellular partial EMT and stemness through increasing EpCAM expression in breast cancer.Cell Signal.2020 Dec;76:109810.doi:10.1016/j.cellsig
MA1067 usage in article: APP:IF, SAMPLE:BREAST CANCER CELLS AND BREAST TISSUE, DILUTION:1:100
Jia X, Wang H,Li Z,Yan J,Guo Y,Zhao W,Gao L,Wang B,Jia Y.HER4 promotes the progression of colorectal cancer by promoting epithelial‑mesenchymal transition.Mol Med Rep.2020 Apr;21(4):1779-1788.doi:10. 3892/mmr.2020.10974.Epub 2020 Feb 4.PMID:32319604;PMCID
MA1067 usage in article: APP:WB, SAMPLE:HCT116 CELL, DILUTION:1:200
Yang Z,Zhang J,Lu D,Sun Y, Zhao X,Wang X,Zhou W,He Q,Jiang Z.Hsa_circ_0137008 suppresses the malignant phenotype in colorectal cancer by acting as a microRNA-338-5p sponge. Cancer Cell Int.2020 Mar 4;20:67. doi:10.1186/s1293 5-020-1150-1.PMID:32158357;PMC
MA1067 usage in article: APP:WB, SAMPLE:SW480 AND HCT116 CELLS, DILUTION: NA
Expression of N%u2011cadherin proteins in myocardial hypertrophy in rats
Expression of N-cadherin in myocardial tissues during the development of a rat heart
Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-beta%u2013mediated epithelial%u2013mesenchymal transition
Ube2v1-mediated ubiquitination and degradation of Sirt1 promotes metastasis of colorectal cancer by epigenetically suppressing autophagy
Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells
MgCl2 and ZnCl2 promote human umbilical vein endothelial cell migration and invasion and stimulate epithelial-mesenchymal transition via the Wnt/?-catenin pathway
Expression of N-cadherin proteins in myocardial hypertrophy in rats
Wnt/?-catenin pathway is required for epithelial to mesenchymal transition in CXCL12 over expressed breast cancer cells
RAC1 overexpression promotes the proliferation, migration and epithelial-mesenchymal transition of lens epithelial cells
MiR-506 inhibits multiple targets in the epithelial-to-mesenchymal transition network and is associated with good prognosis in epithelial ovarian cancer
Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition
Intervention Effects of Ganoderma Lucidum Spores on Epileptiform Discharge Hippocampal Neurons and Expression of Neurotrophin-4 and N-Cadherin
Yang N, Hui L, Wang Y, Yang H, Jiang X. Tumour Biol. 2014 Aug;35(8):7965-73. Doi: 10.1007/S13277-014-2045-3. Epub 2014 May 16. Overexpression Of Sox2 Promotes Migration, Invasion, And Epithelial-Mesenchymal Transition Through The Wnt/??-Catenin Pa...
Tong W, Sun D, Wang Q, Suo J. Cell Biochem Biophys. 2015 Jan 8. [Epub Ahead Of Print] Sorcin Enhances Metastasis And Promotes Epithelial-To-Mesenchymal Transition Of Colorectal Cancer.
Liu H, Liu Z, Jiang B, Peng R, Ma Z, Lu J. Cell Biochem Biophys. 2015 Feb 26. [Epub Ahead Of Print] Sox9 Overexpression Promotes Glioma Metastasis Via Wnt/??-Catenin Signaling.
Kudo-Sakamoto Y, Akazawa H, Ito K, Takano J, Yano M, Yabumoto C, Naito At, Oka T, Lee Jk, Sakata Y, Suzuki J, Saido Tc, Komuro I. J Biol Chem. 2014 Jul 11;289(28):19408-19. Doi: 10.1074/Jbc.M114.567206. Epub 2014 Jun 2. Calpain-Dependent Cleavage ...
Qiao B, Johnson Nw, Gao J. Int J Oncol. 2010 Sep;37(3):663-8. Epithelial-Mesenchymal Transition In Oral Squamous Cell Carcinoma Triggered By Transforming Growth Factor-Beta1 Is Snail Family-Dependent And Correlates With Matrix Metalloproteinase-2 ...
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