|Validated Species:||Chicken, Human, Mouse, Rabbit, Rat|
Data & Images
|Product Name||Anti-Cadherin-2/N-Cadherin Monoclonal Antibody|
|Description||Mouse IgG monoclonal antibody for N-Cadherin, cadherin 2, type 1, N-cadherin (neuronal) (CDH2 ) detection. Tested with WB, IHC-F in Human;mouse;rat;rabbit;chicken. No cross reactivity with other proteins.|
|Cite This Product||Anti-Cadherin-2/N-Cadherin Monoclonal Antibody GC-4 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1067)|
|Replacement Item||This antibody may replace the following items: sc-11743|sc-366034|sc-11746|sc-390336|sc-390147 from Santa Cruz Biotechnology.|
|Validated Species||Chicken, Human, Mouse, Rabbit, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||Affinity purified chicken heart A-CAM.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||99686 MW|
|Protein Function||Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density (By similarity). .|
|Tissue Specificity||In testis, expressed in Sertoli and germ cells. .|
|Subcellular Localization||Cell membrane ; Single-pass type I membrane protein .|
|Alternative Names||Cadherin-2;Neural cadherin;N-cadherin;CD325;Cdh2;|
|Research Areas|||signal transduction|cytoskeleton / ecm|cell adhesion|cadherins||
Background for Cadherin-2
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Cadherin-2/N-Cadherin Monoclonal Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Frozen Section), 4μg/ml, Human, mouse, rat, rabbit, chicken, -|
Western blot, 2μg/ml, Human, mouse, rat, rabbit, chicken
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Cadherin-2/N-Cadherin Monoclonal Antibody Images
Click the images to enlarge.
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Medulla Oblongata Tissue Lysate