radio
SKU MA1067
Size 100μg/vial
Reactivity Chicken, Human, Mouse, Rabbit, Rat
Clonality Monoclonal
Clone Number GC-4
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, WB

Overview

Product Name Anti-Cadherin-2/N-Cadherin Monoclonal Antibody
SKU/Catalog Number MA1067
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for N-Cadherin, cadherin 2, type 1, N-cadherin (neuronal) (CDH2 ) detection. Tested with WB, IHC-F in Human;mouse;rat;rabbit;chicken. No cross reactivity with other proteins.
Cite This Product Anti-Cadherin-2/N-Cadherin Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1067)
Host Mouse
Immunogen Affinity purified chicken heart A-CAM.
Reactivity Chicken, Human, Mouse, Rabbit, Rat

Assay Details

Assay Dilutions Overview

Immunohistochemistry(Frozen Section), 4μg/ml, Human, mouse, rat, rabbit, chicken, -
Western blot, 2μg/ml, Human, mouse, rat, rabbit, chicken

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F).

Images And Assay Conditions

/m/a/ma1067 1_1.jpg

Figure 1. Western blot analysis of Cadherin-2/N-Cadherin using anti- Cadherin-2/N-Cadherin antibody (MA1067).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human PANC-1 whole cell lysates,
Lane 5: human A549 whole cell lysates,
Lane 6: mouse NIH3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- Cadherin-2/N-Cadherin antigen affinity purified monoclonal antibody (Catalog # MA1067) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Cadherin-2/N-Cadherin at approximately 130KD. The expected band size for Cadherin-2/N-Cadherin is at 100KD.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q9Z1Y3
Gene Name Cdh2
Protein Name Cadherin-2
Tissue Specificity In testis, expressed in Sertoli and germ cells. .
Alternative Names Cadherin-2;Neural cadherin;N-cadherin;CD325;Cdh2;
Subcellular Localization Cell membrane ; Single-pass type I membrane protein .
Molecular Weight 99686 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density (By similarity). .
Background N-cadherin(NCAD) is a member of the cadherin cell-cell adhesion receptor family that includes P-, E-, and R-cadherin and liver cell adhesion molecule(L-CAM). N-Cadherin,, also known as Cadherin-2, encodes a 907-amino acid protein that includes a 159-amino acid signal sequence. Human and mouse nucleotide sequences are 96% identical. Mouse Ncad gene consists of 16 exons dispersed over more than 200 kb of genomic DNA. Human N-cadherin gene contains 16 exons and its sequence is highly similar to both the mouse NCAD gene(including the large first and second introns) and other cadherin genes. N-cadherin is mapped to 18q11.2. Cadherin regulates dendritic spine morphogenesis.

Order Product (MA1067)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280
Ships in 5-7 business days.

Troubleshooting

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Publications

Ube2v1-mediated ubiquitination and degradation of Sirt1 promotes metastasis of colorectal cancer by epigenetically suppressing autophagy
Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells
ANXA1 affects cell proliferation, invasion and epithelial-mesenchymal transition of oral squamous cell carcinoma
MgCl2 and ZnCl2 promote human umbilical vein endothelial cell migration and invasion and stimulate epithelial-mesenchymal transition via the Wnt/?-catenin pathway
Expression of N-cadherin proteins in myocardial hypertrophy in rats
Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition
Wnt/?-catenin pathway is required for epithelial to mesenchymal transition in CXCL12 over expressed breast cancer cells
RAC1 overexpression promotes the proliferation, migration and epithelial-mesenchymal transition of lens epithelial cells
MiR-506 inhibits multiple targets in the epithelial-to-mesenchymal transition network and is associated with good prognosis in epithelial ovarian cancer
Intervention Effects of Ganoderma Lucidum Spores on Epileptiform Discharge Hippocampal Neurons and Expression of Neurotrophin-4 and N-Cadherin
Yang N, Hui L, Wang Y, Yang H, Jiang X. Tumour Biol. 2014 Aug;35(8):7965-73. Doi: 10.1007/S13277-014-2045-3. Epub 2014 May 16. Overexpression Of Sox2 Promotes Migration, Invasion, And Epithelial-Mesenchymal Transition Through The Wnt/??-Catenin Pat...
Tong W, Sun D, Wang Q, Suo J. Cell Biochem Biophys. 2015 Jan 8. [Epub Ahead Of Print] Sorcin Enhances Metastasis And Promotes Epithelial-To-Mesenchymal Transition Of Colorectal Cancer.
Liu H, Liu Z, Jiang B, Peng R, Ma Z, Lu J. Cell Biochem Biophys. 2015 Feb 26. [Epub Ahead Of Print] Sox9 Overexpression Promotes Glioma Metastasis Via Wnt/??-Catenin Signaling.
Kudo-Sakamoto Y, Akazawa H, Ito K, Takano J, Yano M, Yabumoto C, Naito At, Oka T, Lee Jk, Sakata Y, Suzuki J, Saido Tc, Komuro I. J Biol Chem. 2014 Jul 11;289(28):19408-19. Doi: 10.1074/Jbc.M114.567206. Epub 2014 Jun 2. Calpain-Dependent Cleavage ...
Qiao B, Johnson Nw, Gao J. Int J Oncol. 2010 Sep;37(3):663-8. Epithelial-Mesenchymal Transition In Oral Squamous Cell Carcinoma Triggered By Transforming Growth Factor-Beta1 Is Snail Family-Dependent And Correlates With Matrix Metalloproteinase-2 ...

Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.