|Validated Species:||Chicken, Human, Mouse, Rabbit, Rat|
Data & Images
|Product Name||Anti-Cadherin-2/N-Cadherin Monoclonal Antibody|
|Description||Mouse IgG monoclonal antibody for N-Cadherin, cadherin 2, type 1, N-cadherin (neuronal) (CDH2 ) detection. Tested with WB, IHC-F in Human;mouse;rat;rabbit;chicken. No cross reactivity with other proteins.|
|Cite This Product||Anti-Cadherin-2/N-Cadherin Monoclonal Antibody GC-4 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1067)|
|Replacement Item||This antibody may replace the following items: sc-11743|sc-366034|sc-11746|sc-390336|sc-390147 from Santa Cruz Biotechnology.|
|Validated Species||Chicken, Human, Mouse, Rabbit, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||Affinity purified chicken heart A-CAM.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||99686 MW|
|Protein Function||Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density (By similarity). .|
|Tissue Specificity||In testis, expressed in Sertoli and germ cells. .|
|Subcellular Localization||Cell membrane ; Single-pass type I membrane protein .|
|Alternative Names||Cadherin-2;Neural cadherin;N-cadherin;CD325;Cdh2;|
|Research Areas|||signal transduction|cytoskeleton / ecm|cell adhesion|cadherins||
Background for Cadherin-2
Anti-Cadherin-2/N-Cadherin Monoclonal Antibody Images
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Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human PANC-1 whole cell lysates,
Lane 5: human A549 whole cell lysates,
Lane 6: mouse NIH3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- Cadherin-2/N-Cadherin antigen affinity purified monoclonal antibody (Catalog # MA1067) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Cadherin-2/N-Cadherin at approximately 130KD. The expected band size for Cadherin-2/N-Cadherin is at 100KD.