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Product Info Summary
| SKU: | A01577-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-N-Cadherin-2 CDH2 CD325-Antibody Picoband®
SKU/Catalog Number
A01577-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-N-Cadherin-2 CDH2 CD325-Antibody Picoband® catalog # A01577-2. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-N-Cadherin-2 CDH2 CD325-Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01577-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Cadherin/CDH2, which shares 95% and 100% amino acid (aa) sequence identity with mouse and rat Cadherin/CDH2, respectively.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01577-2 is reactive to CDH2 in Human
Observed Molecular Weight
140 kDa
Calculated molecular weight
99.8 kDa
Background of CDH2
N-cadherin (NCAD) is a member of the cadherin cell-cell adhesion receptor family that includes P-, E-, and R-cadherin and liver cell adhesion molecule (L-CAM). N-Cadherin,, also known as Cadherin-2, encodes a 907-amino acid protein that includes a 159-amino acid signal sequence. Human and mouse nucleotide sequences are 96% identical. Mouse Ncad gene consists of 16 exons dispersed over more than 200 kb of genomic DNA. Human N-cadherin gene contains 16 exons and its sequence is highly similar to both the mouse NCAD gene (including the large first and second introns) and other cadherin genes. N-cadherin is mapped to 18q11.2. Cadherin regulates dendritic spine morphogenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01577-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Positive Control
WB: human LNCAP whole cell, human SGC-7901 whole cell
Validation Images & Assay Conditions
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Western blot analysis of CDH2 using anti-CDH2 antibody (A01577-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human LNCAP whole cell lysates,
Lane 2: human SGC-7901 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDH2 antigen affinity purified polyclonal antibody (Catalog # A01577-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDH2 at approximately 140KD. The expected band size for CDH2 is at 100KD.
Specific Publications For Anti-N-Cadherin-2 CDH2 CD325-Antibody Picoband® (A01577-2)
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