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CDH2 antibody (A01577-3) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 25 Publications

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a01577 3 cdh2 primary antibodies fcm testing 2

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Flow Cytometry analysis of HEPG2 cells using anti-N Cadherin/CDH2 antibody (A01577-3).
Overlay histogram showing HEPG2 cells stained with A01577-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-N Cadherin/CDH2 Antibody (A01577-3, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Designing a Western blot for CDH2? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the CDH2 Western Blot Guide

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Anti-N Cadherin/CDH2 Antibody Picoband®

Name: Anti-N Cadherin/CDH2 Antibody Picoband®
Brand: Boster Bio
SKU: A01577-3
Price: 370 USD
Availability: InStock
Rating: 100 (1 reviews)

CDH2 antibody

25 Publications|

1 Review

|Submit a Review

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	ELISA, Flow Cytometry, IF, IHC, ICC, WB

Tested Applications

CDH2 antibody was positively detected by WB in:

human A549 whole cell, human HEK293 whole cell, rat liver tissue, mouse liver tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

CDH2 antibody was positively detected by IHC in:

mouse heart tissue, rat heart tissue, human liver cancer tissue, human lung cancer tissue, human pancreatic cancer tissue

Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

CDH2 antibody was positively detected by IF in:

human liver cancer tissue, human liver tissue, mouse heart tissue, rat heart tissue

CDH2 antibody was positively detected by ICC/IF in:

Hela cell

CDH2 antibody was positively detected by FC in:

HEPG2 cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ELISA

Human

Tested

Recommend

Rat

Tested

Recommend

Mouse

Tested

Recommend

Monkey

Predicted

Bovine

Predicted

Sheep

Predicted

Dog

Predicted

Rabbit

Predicted

Product Information

Product Name

Anti-N Cadherin/CDH2 Antibody Picoband®
View all CDH2 Antibodies

Form

Lyophilized

Uniprot

P19022

Gene ID

1000

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human N Cadherin/CDH2 recombinant protein (Position: E170-E266).

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

150 kDa

Calculated Molecular Weight

99.8 kDa

Background of CDH2

N-cadherin (NCAD) is a member of the cadherin cell-cell adhesion receptor family that includes P-, E-, and R-cadherin and liver cell adhesion molecule (L-CAM). N-Cadherin,, also known as Cadherin-2, encodes a 907-amino acid protein that includes a 159-amino acid signal sequence. Human and mouse nucleotide sequences are 96% identical. Mouse Ncad gene consists of 16 exons dispersed over more than 200 kb of genomic DNA. Human N-cadherin gene contains 16 exons and its sequence is highly similar to both the mouse NCAD gene (including the large first and second introns) and other cadherin genes. N-cadherin is mapped to 18q11.2. Cadherin regulates dendritic spine morphogenesis.

Cite This Product

Anti-N Cadherin/CDH2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01577-3)

Protocols

Western Blot protocol for CDH2 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for CDH2 antibody

Review Protocol

Immunofluorescence (IF) protocol for CDH2 antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for CDH2 antibody

Review Protocol

Flow Cytometry (FC) protocol for CDH2 antibody

Review Protocol

Publications

EP300 drives the progression of oral squamous cell carcinoma by regulating NUPR1-mediated ferroptosis and EMT

Published: 2026-08-26

Journal: CLINICAL AND EXPERIMENTAL MEDICINE

Application: WB

Impact Factor: 4.50

Species: Human,Mouse

SKU	A01577-3
Application	Western Blot
Sample	human cervical cancer and adjacent tissues
Sample Processing Description	Three samples each of human cervical cancer and adjacent tissues were collected, and total protein was extracted.
Other Reagents	RIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer
Primary Antibody	N Cadherin/CDH2 Antibody Picoband®
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation	1:10000, 1 h in RT
Detection	Substrate: ECL substrate, Image system: ChemiDoc MP
Results Summary	The purpose of this study was to evaluate the expression differences of N-cadherin in human cervical cancer and adjacent tissues. Results from three samples showed that N-cadherin levels in adjacent tissues were significantly lower than in cancer tissues.

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Anti-N Cadherin/CDH2 Antibody Picoband®

CDH2 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

EP300 drives the progression of oral squamous cell carcinoma by regulating NUPR1-mediated ferroptosis and EMT

YTHDF3 Mediates the Occurrence and Development of Breast Cancer by Regulating Glycolysis Through the mTOR–HIF1α–LHDA Axis

Role of Ca2+‐Dependent Epithelial‐Mesenchymal Transition in Malignant Progression of Colorectal Cancer: Special Focus on REG Iα/EDNRB

SMURF1 Regulates CTCF-HOXA10 Axis and Promotes Tumor Progression in Nasopharyngeal Carcinoma

Taraxasterol Ameliorates Pulmonary Fibrosis by Regulating PPP2R1B Expression

Gut microbiota dysbiosis impairs TGF-β/Smad4 signaling to drive postoperative metastasis in colorectal cancer

ETV1 transcriptional manipulation of KIFC1 regulates the progression of pancreatic cancer

TDH-11 inhibits the proliferation and colonization of colorectal cancer by reducing the activity of HDAC

Ursolic acid, an inhibitor of TMEM16A, co-loaded with cisplatin in hydrogel drug delivery system for multi-targeted therapy of lung cancer

Expression of the laminin genes family and its relationship to prognosis in pancreatic carcinoma

MiR-1290 induces endothelial-to-mesenchymal transition and promotes vascular restenosis after angioplasty by targeting FGF2

Expression and potential molecular mechanism of TOP2A in metastasis of non-small cell lung cancer

PD-L1 knockdown suppresses vasculogenic mimicry of non-small cell lung cancer by modulating ZEB1-triggered EMT

Hepatocyte-targeted delivery using oleanolic acid-loaded liposomes for enhanced hepatocellular carcinoma therapy

Caffeic Acid, an Active Ingredient in Coffee, Combines with DOX for Multitarget Combination Therapy of Lung Cancer

TMEM16A, a Homoharringtonine Receptor, as a Potential Endogenic Target for Lung Cancer Treatment

Inhibition of TMEM16A by Natural Product Silibinin: Potential Lead Compounds for Treatment of Lung Adenocarcinoma

RETRACTED: BMSCs-derived exosomal microRNA-let-7a plays a protective role in diabetic nephropathy via inhibition of USP22 expression

Sohlh2 alleviates malignancy of EOC cells under hypoxia via inhibiting the HIF1α/CA9 signaling pathway

The CXCL12–CXCR4 axis promotes migration, invasiveness, and EMT in human papillary thyroid carcinoma B-CPAP cells via NF-κB signaling

Kallikrein-related peptidase 4 contributes to the tumor metastasis of oral squamous cell carcinoma

Wnt/β-catenin pathway is required for epithelial to mesenchymal transition in CXCL12 over expressed breast cancer cells

RAC1 overexpression promotes the proliferation, migration and epithelial-mesenchymal transition of lens epithelial cells

MiR-506 inhibits multiple targets in the epithelial-to-mesenchymal transition network and is associated with good prognosis in epithelial ovarian cancer

Expression of N‑cadherin proteins in myocardial hypertrophy in rats

Review

Have you used Anti-N Cadherin/CDH2 Antibody Picoband®?

1 Review For Anti-N Cadherin/CDH2 Antibody Picoband®

N Cadherin/CDH2 Antibody Picoband® (A01577-3) shows clear, specific bands in human cervical cancer and adjacent tissues by WB, with higher levels in cancer tissues, demonstrating excellent antibody performance.

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