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Product Info Summary
| SKU: | A15919 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-NACAD Antibody
SKU/Catalog Number
A15919
Size
100μl
Form
Liquid
Description
Boster Bio Anti-NACAD Antibody catalog # A15919. Tested in WB, IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NACAD Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15919)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human NACAD. AA range:376-425
Reactive Species
A15919 is reactive to NACAD in Human, Mouse
Calculated molecular weight
161.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15919 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
Validation Images & Assay Conditions
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Western blot analysis of lysates from Jurkat cells, using NACAD Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HeLa cells using NACAD antibody.
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Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-NACAD Antibody (A15919)
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Customer Q&As
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3 Customer Q&As for Anti-NACAD Antibody
Question
I see that the anti-NACAD antibody A15919 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-02-17
Question
Do you have a BSA free version of anti-NACAD antibody A15919 available?
K. Krishna
Verified customer
Asked: 2016-07-20
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NACAD antibody A15919 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-07-20
Question
We are currently using anti-NACAD antibody A15919 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
P. Jha
Verified customer
Asked: 2014-12-19
Answer
The anti-NACAD antibody (A15919) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-12-19
