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Product Info Summary
| SKU: | A02629-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-NAF1 Antibody Picoband™
SKU/Catalog Number
A02629-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-NAF1 Antibody Picoband™ catalog # A02629-1. Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NAF1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02629-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NAF1 recombinant protein (Position: K173-E340).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02629-1 is reactive to NAF1 in Human
Applications
A02629-1 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
70 kDa
Calculated molecular weight
53.717kDa
Background of NAF1
Enables identical protein binding activity and telomerase RNA binding activity. Involved in regulation of nucleobase-containing compound metabolic process; ribosome biogenesis; and telomerase holoenzyme complex assembly. Located in nucleoplasm. Part of sno(s)RNA-containing ribonucleoprotein complex.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NAF1 using anti-NAF1 antibody (A02629-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human CACO-2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAF1 antigen affinity purified polyclonal antibody (Catalog # A02629-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NAF1 at approximately 70 kDa. The expected band size for NAF1 is at 54 kDa.
Protein Target Info & Infographic
Gene/Protein Information For NAF1 (Source: Uniprot.org, NCBI)
Gene Name
NAF1
Full Name
H/ACA ribonucleoprotein complex non-core subunit NAF1
Weight
53.717kDa
Superfamily
NAF1 family
Alternative Names
nuclear assembly factor 1 homolog (S. cerevisiae) NAF1 nuclear assembly factor 1 ribonucleoprotein H/ACA ribonucleoprotein complex non-core subunit NAF1|nuclear assembly factor 1 homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NAF1, check out the NAF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NAF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NAF1 Antibody Picoband™ (A02629-1)
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No publications found for A02629-1
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Customer Reviews
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