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Product Info Summary
| SKU: | A11849 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Nanog P8 Antibody
SKU/Catalog Number
A11849
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Nanog P8 Antibody catalog # A11849. Tested in WB, IF, ELISA, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Nanog P8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11849)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human NANOGP8. AA range:51-100
Reactive Species
A11849 is reactive to NANOGP8 in Human
Calculated molecular weight
34.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11849 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IF/ICC1:50-200
ELISA 1:2000-20000
IHC 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200 (room temperature, 45min).
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Western blot analysis of lysates from HUVEC, HT-29, HepG2, and Jurkat cells, using NANOGP8 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Nanog P8 Antibody (A11849)
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Customer Q&As
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3 Customer Q&As for Anti-Nanog P8 Antibody
Question
Is a blocking peptide available for product anti-Nanog P8 antibody (A11849)?
B. Johnson
Verified customer
Asked: 2020-05-01
Answer
We do provide the blocking peptide for product anti-Nanog P8 antibody (A11849). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-05-01
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for urinary bladder carcinoma using anti-Nanog P8 antibody A11849. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-01-22
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-22
Question
Would A11849 anti-Nanog P8 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-10-12
Answer
It shows on the product datasheet, A11849 anti-Nanog P8 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-10-12
