Anti-Nanos homolog 1 Nanos1 Antibody

Product Name

Anti-Nanos homolog 1 Nanos1 Antibody

View all NANOS1 Antibodies

SKU/Catalog Number

A07276-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Nanos homolog 1 Nanos1 Antibody (Catalog # A07276-1). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Nanos1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Nanos homolog 1 Nanos1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07276-1)

Host

Rabbit

Contents

Nanos1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Nanos1 antibody was raised against a 14 amino acid synthetic peptide from near the center of human Nanos1. The immunogen is located within amino acids 160 - 210 of Nanos1.

Reactive Species

A07276-1 is reactive to NANOS1 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

30.2 kDa

Background of NANOS1

Nanos1 is one of three known mammalian homologs to the Drosophila gene nanos. Nanos1 is an RNA-binding protein containing a zinc-finger motif and is expressed in the developing nervous system and continues in the adult brain. Interestingly, unlike mice deficient in either nanos2 or nanos3, mice lacking the nanos1 gene develop normally with no sign of abnormalities. Recently it has been found that expression of nanos1 mRNA is down-regulated by E-cadherin in a human breast cancer cell line and the amino-terminal domain on Nanos1 interacts with the E-cadherin-binding protein p120ctn. Furthermore, overexpression of Nanos1 in human colorectal DLD1 cancer cells functionally abolished cell-cell adhesion, allowing the cancer cells to develop strong migratory and invasive properties. These results suggest that targeting Nanos1 might prove an effective strategy in the treatment of E-cadherin-negative tumors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A07276-1 is guaranteed for ELISA, IF, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Nanos1 antibody can be used for detection of Nanos1 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information NANOS1

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