Anti-NAP1L4 Antibody Picoband™

Product Name

Anti-NAP1L4 Antibody Picoband™

View all NAP1L4 Antibodies

SKU/Catalog Number

A08739-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-NAP1L4 Antibody Picoband™ catalog # A08739-1. Tested in WB, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-NAP1L4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08739-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Immunogen

E.coli-derived human NAP1L4 recombinant protein (Position: M1-A331). Human NAP1L4 shares 94% amino acid (aa) sequence identity with rat NAP1L4.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A08739-1 is reactive to NAP1L4 in Human, Mouse, Rat

Applications

A08739-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

65 kDa

Calculated molecular weight

42.823kDa

Background of NAP1L4

Nucleosome assembly protein 1-like 4 is a protein that in humans is encoded by the NAP1L4 gene. This gene encodes a member of the nucleosome assembly protein (NAP) family which can interact with both core and linker histones. It can shuttle between the cytoplasm and nucleus, suggesting a role as a histone chaperone. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of NAP1L4 using anti-NAP1L4 antibody (A08739-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAP1L4 antigen affinity purified polyclonal antibody (Catalog # A08739-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NAP1L4 at approximately 65 kDa. The expected band size for NAP1L4 is at 43 kDa.

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Figure 2. Flow Cytometry analysis of 293T cells using anti-NAP1L4 antibody (A08739-1).
Overlay histogram showing 293T cells stained with A08739-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NAP1L4 Antibody (A08739-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For NAP1L4 (Source: Uniprot.org, NCBI)

Alternative Names

hNAP2; MGC4565; NAP-2; NAP2L; NAP2NAP1L4b; nucleosome assembly protein 1-like 4; nucleosome assembly protein 1-like 4b; Nucleosome assembly protein 2 NAP1L4 NAP1L4b, NAP2, NAP2L, hNAP2 nucleosome assembly protein 1 like 4 nucleosome assembly protein 1-like 4|NAP-2|nucleosome assembly protein 1-like 4b|nucleosome assembly protein 2

For more info on NAP1L4, check out the NAP1L4 Infographic

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In this infographic, you will see the following information for NAP1L4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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