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Product Info Summary
| SKU: | A14492 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-NAP1L5 Antibody
SKU/Catalog Number
A14492
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-NAP1L5 Antibody (Catalog # A14492). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
NAP1L5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-NAP1L5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14492)
Host
Rabbit
Contents
NAP1L5 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
NAP1L5 antibody was raised against an 18 amino acid peptide near the center of human NAP1L5. The immunogen is located within amino acids 40 - 90 of NAP1L5.
Cross-reactivity
NAP1L5 antibody is human, mouse and rat reactive.
Reactive Species
A14492 is reactive to NAP1L5 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
19.6 kDa
Background of NAP1L5
The nucleosome assembly protein 1-like 5 (NAP1L5) protein, also known as DRLM, was initially identified as a possible tumor suppressor gene in hepatocellular carcinoma (1). The NAP1L5 gene is located within the intron of Herc3, and unlike Herc3, is imprinted and highly expressed in regions of the adult mouse brain (2).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14492 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
NAP1L5 antibody can be used for detection of NAP1L5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of NAP1L5 in EL4 cell lysate with NAP1L5 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of NAP1L5 in mouse brain tissue with NAP1L5 antibody at 5 μg/mL.
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Immunofluorescence of NAP1L5 in mouse brain tissue with NAP1L5 antibody at 20 μg/mL.
Specific Publications For Anti-NAP1L5 Antibody (A14492)
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