Anti-NARG1/NAA15 Antibody Picoband™

Product Name

Anti-NARG1/NAA15 Antibody Picoband™

View all NARG1 Antibodies

SKU/Catalog Number

A05636-3

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-NARG1/NAA15 Antibody Picoband™ catalog # A05636-3. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-NARG1/NAA15 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05636-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human NARG1/NAA15 recombinant protein (Position: E249-Y287).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A05636-3 is reactive to Naa15 in Human, Mouse, Rat

Applications

A05636-3 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

100 kDa

Calculated molecular weight

100.961kDa

Background of NARG1

NMDA receptor-regulated protein 1 (NARG1), also known as GA19 or Tbdn100 is a protein that in humans is encoded by the NAA15 gene. It is mapped to chromosome 4. NARG1 is the auxiliary subunit of the NatA (Nα-acetyltransferase A) complex. Both, Naa15 and Naa16 interact with the ribosome in yeast (via the ribosomal proteins, uL23 and uL29), humans and rat, thereby linking the NatA/Naa10 to the ribosome and facilitating co-translational acetylation of nascent polypeptide chains as they emerges from the exit tunnel. Furthermore, Naa15 might act as a scaffold for other factors, including the chaperone like protein HYPK (Huntingtin Interacting Protein K) and Naa50, the catalytic acetyltransferase subunit of NatE.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of NARG1/NAA15 using anti-NARG1/NAA15 antibody (A05636-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NARG1/NAA15 antigen affinity purified polyclonal antibody (Catalog # A05636-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NARG1/NAA15 at approximately 100 kDa. The expected band size for NARG1/NAA15 is at 100,101 kDa.

Gene/Protein Information For Naa15 (Source: Uniprot.org, NCBI)

Alternative Names

FLJ13340; GA19; Gastric cancer antigen Ga19; N(alpha)-acetyltransferase 15, NatA auxiliary subunit; NARG1; NATHGa19; NMDA receptor-regulated protein 1; N-terminal acetyltransferase; Protein tubedown-1; Tbdn100; TBDN100NatA auxiliary subunit; transcriptional coactivator tubedown-100; tubedown-1 Naa15|5730450D16Rik, 6330400I15, AST, ASTBDN, Na, Narg1, Tbdn-, Tbdn-1, mNA, mNAT1, tubedo|N(alpha)-acetyltransferase 15, NatA auxiliary subunit|N-alpha-acetyltransferase 15, NatA auxiliary subunit|N-terminal acetyltransferase 1|N-terminal aceyltransferase 1|NMDA receptor-regulated gene 1|NMDA receptor-regulated protein 1|protein tubedown-1|tubedown

For more info on Naa15, check out the Naa15 Infographic

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In this infographic, you will see the following information for Naa15: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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