Anti-NBPF1/9/10/12/14/15/16/20 Antibody

NBPF12 antibody

Boster Bio Anti-NBPF1/9/10/12/14/15/16/20 Antibody (Catalog # A18001). Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A18001
Size: 100 µl
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-NBPF1/9/10/12/14/15/16/20 Antibody

View all NBPF12 Antibodies

SKU/Catalog Number

A18001

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-NBPF1/9/10/12/14/15/16/20 Antibody (Catalog # A18001). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NBPF1/9/10/12/14/15/16/20 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A18001)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human NBPF1/9/10/12/14/15/16/20.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A18001 is reactive to NBPF12 in Human

Applications

A18001 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

167.689kDa

Background of NBPF12

This gene is a member of the neuroblastoma breakpoint family (NBPF) which consists of dozens of recently duplicated genes primarily located in segmental duplications on human chromosome 1. This gene family has experienced its greatest expansion within the human lineage and has expanded, to a lesser extent, among primates in general. Members of this gene family are characterized by tandemly repeated copies of DUF1220 protein domains. Gene copy number variations in the human chromosomal region 1q21.1, where most DUF1220 domains are located, have been implicated in a number of developmental and neurogenetic diseases such as microcephaly, macrocephaly, autism, schizophrenia, mental retardation, congenital heart disease, neuroblastoma, and congenital kidney and urinary tract anomalies. Altered expression of some gene family members is associated with several types of cancer. This gene family contains numerous pseudogenes.

Gregory S.G., Nature 441:315-321(2006).
Mei G., Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For NBPF12 (Source: Uniprot.org, NCBI)

Gene Name

NBPF12

Full Name

Neuroblastoma breakpoint family member 12

Weight

167.689kDa

Superfamily

NBPF family

Alternative Names

Neuroblastoma breakpoint family member 12 NBPF12 COAS1, KIAA1245 NBPF member 12 neuroblastoma breakpoint family member 12|chomosome one amplified sequence 1 cyclophilin|chromosome 1 amplified sequence 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NBPF12, check out the NBPF12 Infographic

NBPF12 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NBPF12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-NBPF1/9/10/12/14/15/16/20 Antibody

Question

Is there a BSA free version of anti-NBPF1/9/10/12/14/15/16/20 antibody A18001 available?

R. Lewis

Verified customer

Asked: 2019-03-19

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NBPF1/9/10/12/14/15/16/20 antibody A18001 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-03-19

Question

I have a question about product A18001, anti-NBPF1/9/10/12/14/15/16/20 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-07-11

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A18001 anti-NBPF1/9/10/12/14/15/16/20 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-07-11

Question

We are currently using anti-NBPF1/9/10/12/14/15/16/20 antibody A18001 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2018-05-04

Answer

The anti-NBPF1/9/10/12/14/15/16/20 antibody (A18001) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-05-04

Question

Is this A18001 anti-NBPF1/9/10/12/14/15/16/20 antibody reactive to the isotypes of NBPF12?

V. Johnson

Verified customer

Asked: 2016-12-20

Answer

The immunogen of A18001 anti-NBPF1/9/10/12/14/15/16/20 antibody is Synthesized peptide derived from internal of human NBPF1/9/10/12/14/15/16/20. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2016-12-20

Question

I was wanting to use your anti-NBPF1/9/10/12/14/15/16/20 antibody for WB for human body of stomach on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human body of stomach identification?

N. Kulkarni

Verified customer

Asked: 2014-02-10

Answer

It shows on the product datasheet, A18001 anti-NBPF1/9/10/12/14/15/16/20 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human body of stomach in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-02-10

Order DetailsPrice
A18001-100uL

100uL

$360

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A18001
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$360.00

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