Anti-NCAM-L1 L1CAM Monoclonal Antibody

Product Name

Anti-NCAM-L1 L1CAM Monoclonal Antibody

View all L1CAM Antibodies

SKU/Catalog Number

M00729-2

Size

100ul

Form

Liquid

Description

Boster Bio Anti-NCAM-L1 L1CAM Monoclonal Antibody catalog # M00729-2. Tested in WB applications. This antibody reacts with Canine, Human, Mouse, Rabbit, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NCAM-L1 L1CAM Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00729-2)

Host

Mouse

Contents

Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.

Clonality

Monoclonal

Clone Number

1B6

Isotype

IgG

Immunogen

Purified recombinant human NCAM-L1 (N-terminus) protein fragments expressed in E.coli.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

M00729-2 is reactive to L1CAM in Dog, Human, Mouse, Rabbit, Rat

Calculated molecular weight

140.0 kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00729-2 is guaranteed for WB Boster Guarantee

Recommend Dilution

WB 1:1000-1:2000

Anti-TRPC6 monoclonal antibody has been tested by ELISA, immunohistochemistry and western blotting. Expect a band approximately 30 kDa in size corresponding to the cytoplasmic domain of TRPC6 protein by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. Use formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required. Strong staining was observed in adrenal, Purkinje neurons, cortical neurons, heart, ganglion cells, renal tubules, Sertoli cells, hepatocytes, skeletal muscle, exocrine pancreas, and germinal centers of lymphoid follicles. Moderate staining was observed in colon epithelium and plasma cells, B-lymphocytes, and parafollicular cells of the thyroid. Faint staining was seen in respiratory epithelium. Prostate and placenta were negative for staining. The antibody produced minimal to no background staining and appeared very specific at 2.5 µg/mL.

Validation Images & Assay Conditions

See full target information L1CAM

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