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- Table of Contents
L1CAM antibody (M00729-2) performs well in WB and shows reactivity with Dog, Human, Mouse, Rabbit, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Western blotting validation for Anti-NCAM-L1 L1CAM Monoclonal Antibody M00729-2
Western Blot (WB) analysis using NCAM-L1 Monoclonal Antibody against HepG2 cell lysate.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
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Anti-NCAM-L1 L1CAM Monoclonal Antibody
L1CAM antibody
| Reactive Species | Host | Application |
|---|---|---|
| Dog, Human, Mouse, Rabbit, Rat | Mouse | WB |
Recommend Dilution
Application
Dilution
Species
WB 1:1000-1:2000
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Anti-TRPC6 monoclonal antibody has been tested by ELISA, immunohistochemistry and western blotting. Expect a band approximately 30 kDa in size corresponding to the cytoplasmic domain of TRPC6 protein by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. Use formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required. Strong staining was observed in adrenal, Purkinje neurons, cortical neurons, heart, ganglion cells, renal tubules, Sertoli cells, hepatocytes, skeletal muscle, exocrine pancreas, and germinal centers of lymphoid follicles. Moderate staining was observed in colon epithelium and plasma cells, B-lymphocytes, and parafollicular cells of the thyroid. Faint staining was seen in respiratory epithelium. Prostate and placenta were negative for staining. The antibody produced minimal to no background staining and appeared very specific at 2.5 µg/mL.
Product Information
Product Name
Anti-NCAM-L1 L1CAM Monoclonal Antibody
View all L1CAM Antibodies
View all L1CAM Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Clonality
Monoclonal
Clone Number
1B6
Isotype
IgG
Immunogen
Purified recombinant human NCAM-L1 (N-terminus) protein fragments expressed in E.coli.
Cross-Reactivity
No cross reactivity with other proteins.
Calculated Molecular Weight
140.0 kDa
Cite This Product
Anti-NCAM-L1 L1CAM Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00729-2)
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Product Promise
We promise Anti-NCAM-L1 L1CAM Monoclonal Antibody performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
M00729-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$380.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 10, 2026