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Product Info Summary
| SKU: | M00184-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-NCAM NCAM1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00184-1
BM4951 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-NCAM NCAM1 Rabbit Monoclonal Antibody catalog # M00184-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NCAM NCAM1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00184-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AOGF-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human NCAM
Reactive Species
M00184-1 is reactive to NCAM1 in Human
Observed Molecular Weight
180 kDa
Calculated molecular weight
94.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00184-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:100
Positive Control
WB: SH-SY5Y cell
Validation Images & Assay Conditions
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Western blot analysis of NCAM expression in SH-SY5Y cell lysate.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Specific Publications For Anti-NCAM NCAM1 Rabbit Monoclonal Antibody (M00184-1)
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Customer Q&As
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4 Customer Q&As for Anti-NCAM NCAM1 Rabbit Monoclonal Antibody
Question
We have seen staining in human testis. What should we do? Is anti-NCAM Rabbit Monoclonal antibody supposed to stain testis positively?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
According to literature testis does express NCAM1. According to Uniprot.org, NCAM1 is expressed in neocortex, skeletal muscle, testis, brain, brain, kidney ovary, plasma, liver, among other tissues. Regarding which tissues have NCAM1 expression, here are a few articles citing expression in various tissues:
Brain, Kidney, and Ovary, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Plasma, Pubmed ID: 16335952
Skeletal muscle, Pubmed ID: 2887295, 3253057
Boster Scientific Support
Answered: 2019-11-18
Question
We ordered your anti-NCAM Rabbit Monoclonal antibody for IF on brain a few years ago. I am using human, and We intend to use the antibody for WB next. I am looking for examining brain as well as skeletal muscle in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-05-06
Answer
I viewed the website and datasheets of our anti-NCAM Rabbit Monoclonal antibody and I see that M00184-1 has been validated on human in both IF and WB. Thus M00184-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-05-06
Question
We are currently using anti-NCAM Rabbit Monoclonal antibody M00184-1 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2018-06-27
Answer
The anti-NCAM Rabbit Monoclonal antibody (M00184-1) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-27
Question
My team were well pleased with the WB result of your anti-NCAM Rabbit Monoclonal antibody. However we have observed positive staining in kidney ovary isoform 1: cell membrane using this antibody. Is that expected? Could you tell me where is NCAM1 supposed to be expressed?
A. Moore
Verified customer
Asked: 2014-04-02
Answer
According to literature, kidney ovary does express NCAM1. Generally NCAM1 expresses in isoform 1: cell membrane. Regarding which tissues have NCAM1 expression, here are a few articles citing expression in various tissues:
Brain, Kidney, and Ovary, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Plasma, Pubmed ID: 16335952
Skeletal muscle, Pubmed ID: 2887295, 3253057
Boster Scientific Support
Answered: 2014-04-02
