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Product Info Summary
| SKU: | A07424-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, ICC, WB |
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Product info
Product Name
Anti-NCBP1 Antibody
SKU/Catalog Number
A07424-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-NCBP1 Antibody (Catalog # A07424-1). Tested in ELISA, WB, ICC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
NCBP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-NCBP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07424-1)
Host
Rabbit
Contents
NCBP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
NCBP1 antibody was raised against a 17 amino acid peptide near the center of human NCBP1. The immunogen is located within amino acids 420 - 470 of NCBP1.
Cross-reactivity
NCBP1 antibody is human, mouse and rat reactive. At least two isoforms of NCBP1 are known to exist; this antibody will detect both isoforms of NCBP1.
Reactive Species
A07424-1 is reactive to NCBP1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
91.8 kDa
Background of NCBP1
NCBP1, also known as CBP80, is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. NCBP1 promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay (1,2). Recent evidence has shown that cellular-cap-binding proteins such as NCBP1 associate with influenza virus mRNAs, suggesting that these viral mRNAs may follow the normal cellular pathways for splicing, nuclear export, and translation (3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07424-1 is guaranteed for ELISA, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
NCBP1 antibody can be used for detection of NCBP1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of NCBP1 in HeLa cell lysate with NCBP1 antibody at (A) 1 and (B) 2 μg/mL.
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Immunocytochemistry of NCBP1 in HeLa cells with NCBP1 antibody at 2.5 μg/ml.
Specific Publications For Anti-NCBP1 Antibody (A07424-1)
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