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Product Info Summary
| SKU: | M01731-1 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-NDC80 Antibody (C-Term)
SKU/Catalog Number
M01731-1
Size
50 µl
Description
Boster Bio Anti-NDC80 Antibody (C-Term) (Catalog # M01731-1). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-NDC80 Antibody (C-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01731-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This NDC80 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 594-628 amino acids from human NDC80.
Reactive Species
M01731-1 is reactive to NDC80 in Human
Calculated molecular weight
73.9 kDa
Background of NDC80
Acts as a component of the essential kinetochore- associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01731-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
Validation Images & Assay Conditions
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All lanes : Anti-NDC80 Antibody (C-Term) at 1:2000 dilution
Lane 1: Hela whole cell lysate
Lane 2: MCF-7 whole cell lysate
Lane 3: U-2OS whole cell lysate
Lane 4: K562 whole cell lysate
Lane 5: 293T/17 whole cell lysate
Lane 6: Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 74 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-NDC80 Antibody (C-Term) (M01731-1)
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