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Product Info Summary
| SKU: | A08570 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-NDST2 Antibody
View all N-Deacetylase/N-Sulfotransferase 2/NDST2 Antibodies
SKU/Catalog Number
A08570
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-NDST2 Antibody (Catalog # A08570). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
NDST2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-NDST2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08570)
Host
Rabbit
Contents
NDST2 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
NDST2 antibody was raised against a 16 amino acid peptide near the amino terminus of human NDST2. The immunogen is located within amino acids 20 - 70 of NDST2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
NDST2 antibody is human, mouse and rat reactive. At least two isoforms of NDST2 are known to exist; this antibody will only detect the larger isoform. NDST2 antibody is predicted not to cross-react with other members of the NDST protein family.
Reactive Species
A08570 is reactive to NDST2 in Human, Mouse, Rat
Applications
A08570 is guaranteed for ELISA, IF, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
100.875kDa
Background of N-Deacetylase/N-Sulfotransferase 2/NDST2
The N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 2 (NDST2) protein, also known as N-heparan sulfate sulfotransferase 2, and the highly related protein NDST1 have key roles in the sulfation of heparan sulfate proteoglycans, molecules that are present of cell surfaces and the extracellular matrix (1,2). NDST1 and NDST2 are dispensable for mesodermal differentiation into osteoblasts but necessary for induction of adipocytes and neural cells (3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
NDST2 antibody can be used for detection of PDCL by Western blot at 1 - 2 μg/ml. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of NDST2 in A-20 cell lysate with NDST2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunofluorescence of NDST2 in Daudi cells with NDST2 antibody at 20 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For NDST2 (Source: Uniprot.org, NCBI)
Gene Name
NDST2
Full Name
Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2
Weight
100.875kDa
Superfamily
sulfotransferase 1 family
Alternative Names
bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2; EC 2.8.2.8; Glucosaminyl N-deacetylase/N-sulfotransferase 2; HSST2MGC129697; MGC129705; N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 2; NDST2; NDST-2; N-heparan sulfate sulfotransferase 2; N-HSST 2; NST2 NDST2 HSST2, N-HSST 2, NST2 N-deacetylase and N-sulfotransferase 2 bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2|N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 2|N-heparan sulfate sulfotransferase 2|glucosaminyl N-deacetylase/N-sulfotransferase 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NDST2, check out the NDST2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NDST2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NDST2 Antibody (A08570)
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Customer Reviews
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