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Product Info Summary
| SKU: | A11694-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-NDUFA3 Antibody Picoband®
SKU/Catalog Number
A11694-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-NDUFA3 Antibody Picoband® catalog # A11694-1. Tested in ELISA, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NDUFA3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11694-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NDUFA3 recombinant protein (Position: M1-L84).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A11694-1 is reactive to NDUFA3 in Human, Rat
Observed Molecular Weight
9 kDa
Calculated molecular weight
9.3 kDa
Background of NDUFA3
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 is a protein that in humans is encoded by the NDUFA3 gene. Involved in mitochondrial respiratory chain complex I assembly. Located in mitochondrion. Part of mitochondrial respiratory chain complex I.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11694-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat heart tissue
Validation Images & Assay Conditions
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Western blot analysis of NDUFA3 using anti-NDUFA3 antibody (A11694-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFA3 antigen affinity purified polyclonal antibody (Catalog # A11694-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFA3 at approximately 9 kDa. The expected band size for NDUFA3 is at 9 kDa.
Specific Publications For Anti-NDUFA3 Antibody Picoband® (A11694-1)
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