Anti-NDUFA8 Antibody Picoband™

Product Name

Anti-NDUFA8 Antibody Picoband™

View all NDUFA8 Antibodies

SKU/Catalog Number

A10547-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-NDUFA8 Antibody Picoband™ catalog # A10547-2. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-NDUFA8 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10547-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human NDUFA8 recombinant protein (Position: M1-K172).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A10547-2 is reactive to NDUFA8 in Human, Mouse, Rat

Applications

A10547-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

19 kDa

Calculated molecular weight

20.105kDa

Background of NDUFA8

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 is an enzyme that in humans is encoded by the NDUFA8 gene. The protein encoded by this gene belongs to the complex I 19 kDa subunit family. Mammalian complex I is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It plays an important role in transfering electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of NDUFA8 using anti-NDUFA8 antibody (A10547-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: rat heart tissue lysates,
Lane 4: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFA8 antigen affinity purified polyclonal antibody (Catalog # A10547-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFA8 at approximately 19 kDa. The expected band size for NDUFA8 is at 20 kDa.

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Figure 2. Flow Cytometry analysis of 293T cells using anti-NDUFA8 antibody (A10547-2).
Overlay histogram showing 293T cells stained with A10547-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFA8 Antibody (A10547-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Figure 3. Flow Cytometry analysis of U87 cells using anti-NDUFA8 antibody (A10547-2).
Overlay histogram showing U87 cells stained with A10547-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFA8 Antibody (A10547-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For NDUFA8 (Source: Uniprot.org, NCBI)

Alternative Names

CI-19KD; CI-PGIV; complex I PGIV subunit; Complex I-19kD; Complex I-PGIV; MGC793; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8 (19kD, PGIV); NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8, 19kDa; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8; NADH:ubiquinone oxidoreductase PGIV subunit; NADH-ubiquinone oxidoreductase 19 kDa subunit; PGIV NDUFA8 CI-19KD, CI-PGIV, PGIV NADH:ubiquinone oxidoreductase subunit A8 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8|NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8, 19kDa|NADH-ubiquinone oxidoreductase 19 kDa subunit|NADH:ubiquinone oxidoreductase PGIV subunit|complex I-19kD|complex I-PGIV

For more info on NDUFA8, check out the NDUFA8 Infographic

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In this infographic, you will see the following information for NDUFA8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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