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Product Info Summary
| SKU: | A03608-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IHC, WB |
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Product info
Product Name
Anti-Ndufs4 Antibody
SKU/Catalog Number
A03608-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Ndufs4 Antibody catalog # A03608-1. Tested in WB,IP,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ndufs4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03608-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Full length recombinant protein.
Reactive Species
A03608-1 is reactive to NDUFS4 in Human, Mouse, Rat
Calculated molecular weight
20.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03608-1 is guaranteed for Flow Cytometry, IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1,000
IP: 1:10-1:50
IHC: 1:50-1:200
FC: 1:50-1:100
Validation Images & Assay Conditions
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Western blot analysis of Ndufs4 on rat heart tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-Ndufs4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with ET7109-37 at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
Specific Publications For Anti-Ndufs4 Antibody (A03608-1)
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