Anti-NDUFS5 Antibody

NDUFS5 antibody

Boster Bio Anti-NDUFS5 Antibody (Catalog # A04093). Tested in WB, IHC applications. This antibody reacts with Human.

Product Info Summary

SKU: A04093
Size: 100 µl
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

Product Name

Anti-NDUFS5 Antibody

View all NDUFS5 Antibodies

SKU/Catalog Number

A04093

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-NDUFS5 Antibody (Catalog # A04093). Tested in WB, IHC applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NDUFS5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04093)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human NDUFS5.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04093 is reactive to NDUFS5 in Human

Applications

A04093 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

12.518kDa

Background of NDUFS5

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Mao M., Proc. Natl. Acad. Sci. U.S.A. 95:8175-8180(1998).
Loeffen J., J. Inherit. Metab. Dis. 22:19-28(1999).<

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Validation Images & Assay Conditions

Gene/Protein Information For NDUFS5 (Source: Uniprot.org, NCBI)

Gene Name

NDUFS5

Full Name

NADH dehydrogenase [ubiquinone] iron-sulfur protein 5

Weight

12.518kDa

Superfamily

complex I NDUFS5 subunit family

Alternative Names

CI-15 kDa; CI15K; CI-15k; Complex I-15 kDa; NADH dehydrogenase (ubiquinone) Fe-S protein 5 (15kD) (NADH-coenzyme Qreductase); NADH dehydrogenase (ubiquinone) Fe-S protein 5, 15kDa (NADH-coenzyme Qreductase); NADH dehydrogenase [ubiquinone] iron-sulfur protein 5; NADH:ubiquinone oxidoreductase 15 kDa IP subunit; NADH-ubiquinone oxidoreductase 15 kDa subunit NDUFS5 CI-15k, CI15K NADH:ubiquinone oxidoreductase subunit S5 NADH dehydrogenase [ubiquinone] iron-sulfur protein 5|CI-15 kDa|NADH dehydrogenase (ubiquinone) Fe-S protein 5, 15kDa (NADH-coenzyme Q reductase)|NADH:ubiquinone oxidoreductase 15 kDa IP subunit|complex I-15 kDa

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NDUFS5, check out the NDUFS5 Infographic

NDUFS5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NDUFS5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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13 Customer Q&As for Anti-NDUFS5 Antibody

Question

My question regarding product A04093, anti-NDUFS5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-04-06

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04093 anti-NDUFS5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-04-06

Question

Is this A04093 anti-NDUFS5 antibody reactive to the isotypes of NDUFS5?

Verified Customer

Verified customer

Asked: 2020-03-27

Answer

The immunogen of A04093 anti-NDUFS5 antibody is Synthesized peptide derived from internal of human NDUFS5. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-03-27

Question

Will anti-NDUFS5 antibody A04093 work on pig WB with liver?

Verified Customer

Verified customer

Asked: 2020-03-06

Answer

Our lab technicians have not tested anti-NDUFS5 antibody A04093 on pig. You can run a BLAST between pig and the immunogen sequence of anti-NDUFS5 antibody A04093 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig liver in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-03-06

Question

I was wanting to use your anti-NDUFS5 antibody for WB for human umbilical cord blood on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human umbilical cord blood identification?

Verified Customer

Verified customer

Asked: 2019-11-04

Answer

You can see on the product datasheet, A04093 anti-NDUFS5 antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human umbilical cord blood in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-11-04

Question

I have attached the WB image, lot number and protocol we used for umbilical cord blood using anti-NDUFS5 antibody A04093. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-10-28

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-28

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for umbilical cord blood using anti-NDUFS5 antibody A04093. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-09-17

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-17

Question

Do you have a BSA free version of anti-NDUFS5 antibody A04093 available?

Verified Customer

Verified customer

Asked: 2019-09-03

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NDUFS5 antibody A04093 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-09-03

Question

Would A04093 anti-NDUFS5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-05-13

Answer

As indicated on the product datasheet, A04093 anti-NDUFS5 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-05-13

Question

Would anti-NDUFS5 antibody A04093 work for WB with umbilical cord blood?

M. Wu

Verified customer

Asked: 2018-11-14

Answer

According to the expression profile of umbilical cord blood, NDUFS5 is highly expressed in umbilical cord blood. So, it is likely that anti-NDUFS5 antibody A04093 will work for WB with umbilical cord blood.

Boster Scientific Support

Answered: 2018-11-14

Question

Is a blocking peptide available for product anti-NDUFS5 antibody (A04093)?

T. Jackson

Verified customer

Asked: 2018-11-01

Answer

We do provide the blocking peptide for product anti-NDUFS5 antibody (A04093). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-11-01

Question

I am interested in to test anti-NDUFS5 antibody A04093 on human umbilical cord blood for research purposes, then I may be interested in using anti-NDUFS5 antibody A04093 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-02-23

Answer

The products we sell, including anti-NDUFS5 antibody A04093, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-02-23

Question

I see that the anti-NDUFS5 antibody A04093 works with WB, what is the protocol used to produce the result images on the product page?

W. Parker

Verified customer

Asked: 2017-12-15

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-12-15

Question

We are currently using anti-NDUFS5 antibody A04093 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?

K. Wu

Verified customer

Asked: 2016-09-30

Answer

The anti-NDUFS5 antibody (A04093) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-09-30

Order DetailsPrice
A04093-100uL

100uL

$360

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A04093
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