Anti-NDUFS5 Antibody (N-Term)

Product Name

Anti-NDUFS5 Antibody (N-Term)

View all NDUFS5 Antibodies

SKU/Catalog Number

M04093

Size

50 µl

Description

Boster Bio Anti-NDUFS5 Antibody (N-Term) (Catalog # M04093). Tested in WB application(s). This antibody reacts with Human.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-NDUFS5 Antibody (N-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04093)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This NDUFS5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 5-36 amino acids from human NDUFS5.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M04093 is reactive to NDUFS5 in Human

Applications

M04093 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

12.518kDa

Background of NDUFS5

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:2000

Validation Images & Assay Conditions

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All lanes : Anti-NDUFS5 Antibody (N-Term) at 1:2000 dilution
Lane 1: human kidney lysate
Lane 2: human liver lysate
Lane 3: Raji whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 13 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Gene/Protein Information For NDUFS5 (Source: Uniprot.org, NCBI)

Alternative Names

CI-15 kDa; CI15K; CI-15k; Complex I-15 kDa; NADH dehydrogenase (ubiquinone) Fe-S protein 5 (15kD) (NADH-coenzyme Qreductase); NADH dehydrogenase (ubiquinone) Fe-S protein 5, 15kDa (NADH-coenzyme Qreductase); NADH dehydrogenase [ubiquinone] iron-sulfur protein 5; NADH:ubiquinone oxidoreductase 15 kDa IP subunit; NADH-ubiquinone oxidoreductase 15 kDa subunit NDUFS5 CI-15k, CI15K NADH:ubiquinone oxidoreductase subunit S5 NADH dehydrogenase [ubiquinone] iron-sulfur protein 5|CI-15 kDa|NADH dehydrogenase (ubiquinone) Fe-S protein 5, 15kDa (NADH-coenzyme Q reductase)|NADH:ubiquinone oxidoreductase 15 kDa IP subunit|complex I-15 kDa

For more info on NDUFS5, check out the NDUFS5 Infographic

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In this infographic, you will see the following information for NDUFS5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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