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Product Info Summary
| SKU: | M05146 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-NDUFV1 Antibody (Center)
SKU/Catalog Number
M05146
Size
50 µl
Description
Boster Bio Anti-NDUFV1 Antibody (Center) (Catalog # M05146). Tested in WB application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-NDUFV1 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05146)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This NDUFV1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 194-226 amino acids from the Central region of human NDUFV1.
Reactive Species
M05146 is reactive to NDUFV1 in Human, Mouse
Calculated molecular weight
50.8 kDa
Background of NDUFV1
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05146 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
Validation Images & Assay Conditions
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All lanes : Anti-NDUFV1 Antibody (Center) at 1:2000 dilution
Lane 1: mouse kidney lysate
Lane 2: HepG2 whole cell lysate
Lane 3: A431 whole cell lysate
Lane 4: HL-60 whole cell lysate
Lane 5: HT-29 whole cell lysate
Lane 6: mouse brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 51 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-NDUFV1 Antibody (Center) (M05146)
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