Anti-NEDD4-binding protein 1 N4BP1 Antibody

Product Name

Anti-NEDD4-binding protein 1 N4BP1 Antibody

View all N4BP1 Antibodies

SKU/Catalog Number

A12575-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-NEDD4-binding protein 1 N4BP1 Antibody (Catalog # A12575-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

N4BP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-NEDD4-binding protein 1 N4BP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12575-1)

Host

Rabbit

Contents

N4BP1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

N4BP1 antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human N4BP1. The immunogen is located within the first 50 amino acids of N4BP1.

Cross-reactivity

At least three isoforms of N4BP1 are known to exist; this antibody will only detect the longest isoform.

Reactive Species

A12575-1 is reactive to N4BP1 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

100.4 kDa

Background of N4BP1

Nedd4-binding partner-1 (N4BP1) is a developmentally expressed protein interactor and monoubiquitylation substrate of Nedd4, a HECT domain-containing ubiquitin ligase that mediates ubiquitylation and proteasome degradation of target proteins and may have a fundamental role to play in embryonic processes. N4BP1 can undergo Nedd4-mediated polyubiquitylation and proteosomal degradation. N4BP1 also functions as a negative regulator of AIF4 (also known as ITCH or Atrophin-1), an E3-ubiquitin ligase, by interacting with the second WW domain of AIF4 and impairing ubiquitination of substrates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A12575-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

N4BP1 antibody can be used for detection of N4BP1 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information N4BP1

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