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- Table of Contents
N4BP1 antibody (A12575-1) performs well in ELISA, IF, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescence of N4BP1 in HeLa cells with N4BP1 antibody at 20 μg/mL.
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Anti-NEDD4-binding protein 1 N4BP1 Antibody
N4BP1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, ICC, WB |
Recommend Dilution
Application
Dilution
Species
N4BP1 antibody can be used for detection of N4BP1 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-NEDD4-binding protein 1 N4BP1 Antibody
View all N4BP1 Antibodies
View all N4BP1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
N4BP1 antibody can be stored at 4°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Host
Rabbit
Contents
N4BP1 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
N4BP1 antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human N4BP1. The immunogen is located within the first 50 amino acids of N4BP1.
Cross-Reactivity
At least three isoforms of N4BP1 are known to exist; this antibody will only detect the longest isoform.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
100.4 kDa
Background of N4BP1
Nedd4-binding partner-1 (N4BP1) is a developmentally expressed protein interactor and monoubiquitylation substrate of Nedd4, a HECT domain-containing ubiquitin ligase that mediates ubiquitylation and proteasome degradation of target proteins and may have a fundamental role to play in embryonic processes. N4BP1 can undergo Nedd4-mediated polyubiquitylation and proteosomal degradation. N4BP1 also functions as a negative regulator of AIF4 (also known as ITCH or Atrophin-1), an E3-ubiquitin ligase, by interacting with the second WW domain of AIF4 and impairing ubiquitination of substrates.
Cite This Product
Anti-NEDD4-binding protein 1 N4BP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12575-1)
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Product Promise
We promise Anti-NEDD4-binding protein 1 N4BP1 Antibody performs excellently in relevant applications for ELISA, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A12575-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026