Product Info Summary
SKU: | A12575-1 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-NEDD4-binding protein 1 N4BP1 Antibody
SKU/Catalog Number
A12575-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-NEDD4-binding protein 1 N4BP1 Antibody (Catalog # A12575-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
N4BP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-NEDD4-binding protein 1 N4BP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12575-1)
Host
Rabbit
Contents
N4BP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
N4BP1 antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human N4BP1. The immunogen is located within the first 50 amino acids of N4BP1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three isoforms of N4BP1 are known to exist; this antibody will only detect the longest isoform.
Reactive Species
A12575-1 is reactive to N4BP1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
100379 MW
Background of N4BP1
Nedd4-binding partner-1 (N4BP1) is a developmentally expressed protein interactor and monoubiquitylation substrate of Nedd4, a HECT domain-containing ubiquitin ligase that mediates ubiquitylation and proteasome degradation of target proteins and may have a fundamental role to play in embryonic processes. N4BP1 can undergo Nedd4-mediated polyubiquitylation and proteosomal degradation. N4BP1 also functions as a negative regulator of AIF4 (also known as ITCH or Atrophin-1), an E3-ubiquitin ligase, by interacting with the second WW domain of AIF4 and impairing ubiquitination of substrates.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12575-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
N4BP1 antibody can be used for detection of N4BP1 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of N4BP1 in HeLa cell lysate with N4BP1 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunocytochemistry of N4BP1 in HeLa cells with N4BP1 antibody at 10 μg/mL.
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Immunofluorescence of N4BP1 in HeLa cells with N4BP1 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For N4BP1 (Source: Uniprot.org, NCBI)
Gene Name
N4BP1
Full Name
NEDD4-binding protein 1
Weight
100379 MW
Superfamily
N4BP1 family
Alternative Names
KIAA0615, NEDD4-binding protein 1, N4BP1 N4BP1 NEDD4 binding protein 1 NEDD4-binding protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on N4BP1, check out the N4BP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for N4BP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NEDD4-binding protein 1 N4BP1 Antibody (A12575-1)
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