|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-CD11b Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for Integrin alpha-M(ITGAM) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-CD11b Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9140)|
|Replacement Item||This antibody may replace the following items: sc-1186|sc-20050|sc-23934|sc-23937|sc-28664|sc-51657|sc-59744|sc-65429|sc-6612|sc-71444|sc-80542|sc-81721|sc-81723 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||E.coli-derived human CD11b recombinant protein (Position: F17-T382). Human CD11b shares 79% and 61% amino acid (aa) sequences identity with mouse and rat CD11b, respectively.|
|Cross Reactivity||No cross reactivity with other proteins.|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Integrin alpha-M|
|Molecular Weight||127179 MW|
|Protein Function||Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.|
|Tissue Specificity||Predominantly expressed in monocytes and granulocytes.|
|Sequence Similarities||Belongs to the integrin alpha chain family.|
|Subcellular Localization||Membrane; Single-pass type I membrane protein.|
|Alternative Names||Integrin alpha-M;CD11 antigen-like family member B;CR-3 alpha chain;Cell surface glycoprotein MAC-1 subunit alpha;Leukocyte adhesion receptor MO1;Neutrophil adherence receptor;CD11b;ITGAM;CD11B, CR3A;|
Background for Integrin alpha-M
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-CD11b Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat|
Western blot, 0.1-0.5μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-CD11b Picoband™ Antibody Images
Click the images to enlarge.
All lanes: Anti CD11B (PB9140) at 0.5ug/ml
WB: Recombinant Human CD11B Protein 0.5ng
Predicted bind size: 45KD
Observed bind size: 45KD
IHC(P): Human Tonsil Tissue
IHC(P): Mouse Spleen Tissue
IHC(P): Rat Spleen Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,