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Anti-NEFH Antibody (Monoclonal, N52)

Boster Bio Anti-NEFH Antibody (Monoclonal, N52) catalog # MA1071. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100μg/vial in Lyophilized form antibody. Cited in 12 publication(s).

Publications (12)
Q&As (5)
Datasheet
Protocols
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Figure 1. IHC analysis of NEFH using anti-NEFH antibody (MA1071).

Figure 2. Western blot analysis of NEFH using anti-NEFH antibody (MA1071).

Anti-NEFH Antibody (Monoclonal, N52) Product Summary

SKU:	MA1071
Size:	100μg/vial
Reactivity:	Human, Mouse, Rat
Clonality:	Monoclonal
Clone Number:	N52
Host:	Mouse
Applications:	IHC, WB
Conjugation:	Choose a conjugate option when adding to cart. Sample size cannot be ordered with conjugation. Conjugated antibody size will be reduced in half due to loss in the conjugation process.

Product Info

Overview

Product Name	Anti-NEFH Antibody (Monoclonal, N52) See more NF-H products
Catalog#	MA1071
Pack Size	100μg/vial
Storage & Handling	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Description	Boster Bio Anti-NEFH Antibody (Monoclonal, N52) catalog # MA1071. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100μg/vial in Lyophilized form antibody.
Cite This Product	Anti-NEFH Antibody (Monoclonal, N52) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1071)
Antibodies Validation	Antibodies Validation Information
Similar Products From Other Companies	Anti-NEFH Antibody (Monoclonal, N52) may replace the following items: sc 133165\|sc 133237\|sc 137009\|sc 20014\|sc 20015\|sc 20112\|sc 22909\|sc 32273\|sc 32728\|sc 32729\|sc 32730\|sc 51682\|sc 52350\|sc 53798\|sc 56575\|sc 58553.

Product Specs

Host	Mouse
Reactive Species	Human, Mouse, Rat
Applications	IHC, WB *Our Boster Guarantee covers the use of this product in the above tested applications. *Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
Related Products	Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F). Blocking peptide can be purchased at $100. Contact us for more information. *Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
Immunogen	C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200.
Cross Reactivity	No cross reactivity with other proteins

Protein Target Info

Gene/Protein Basic Information For Nefh (Source: Uniprot.org, NCBI)

Uniprot Id	P16884
NCBI Gene Id	24587
Species Of This Entry	Rat
Gene Name	Nefh
Protein Name	Neurofilament heavy polypeptide
Superfamily	intermediate filament family
Alternative Names	NF-H\|200 kDa neurofilament protein; KIAA0845; NEFH; Neurofilament Heavy (200kDa); neurofilament heavy polypeptide; Neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa; neurofilament, heavy polypeptide; NF200; NFH; NF-H
Molecular Weight	115378

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Nefh, check out the Nefh Infographic

We have 30,000+ of these available, one for each gene! Check them out.

Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.

In this infographic you will see the following information for Nefh: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.

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Assay Dilutions Overview

Our Boster Quality Guarantee for Anti-NEFH Antibody (Monoclonal, N52) covers its use in the following applications.

Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, By Heat
Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, mouse, rat, -
Western blot, 0.5ml, Human, mouse, rat

*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.

Images & QC Assay Conditions

Figure 1. IHC analysis of NEFH using anti-NEFH antibody (MA1071).
NEFH was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-NEFH Antibody (MA1071) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Figure 2. Western blot analysis of NEFH using anti-NEFH antibody (MA1071).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-NEFH antigen affinity purified monoclonal antibody (Catalog # MA1071)) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for NEFH at approximately 200KD. The expected band size for NEFH is at 112KD.

Specific Protocols

Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.

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Publications

Total number of citations: 12

Wu K,Yue J,Shen K,He J,Zhu G,Liu S,Zhang C,Yang H. Increased Expression of Fibroblast Growth Factor 13 in Cortical Lesions of the Focal Cortical Dysplasia. Brain Res Bull. 2020 Dec 4:S0361-9230(20)30710-3.doi: 10.1016/j.brainresbull.2020.11.023.Epub ahead Species: Human MA1071 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:50	PubMed ID 33285262
Changes in the Expression of miR-34a and its Target Genes Following Spinal Cord Injury In Rats	PubMed ID 27780189
MicroRNA-30b regulates expression of the sodium channel Nav17 in nerve injury-induced neuropathic pain in the rat	PubMed ID 27765894
Neuron-derived IgG Protects Neurons from Complement-dependent Cytotoxicity	PubMed ID 23979841
An Acetylcholinesterase Antibody-Based Quartz Crystal Microbalance for the Rapid Identification of Spinal Ventral and Dorsal Roots	PubMed ID 23935920
Trimebutine, a small molecule mimetic agonist of adhesion molecule L1, contributes to functional recovery after spinal cord injury in mice	PubMed ID 28714852
Expression of pannexin 1 and 2 in cortical lesions from intractable epilepsy patients with focal cortical dysplasia	PubMed ID 28036289
Downregulation of CD47 and CD200 in patients with focal cortical dysplasia type IIb and tuberous sclerosis complex	PubMed ID 27095555
Lentiviral-mediated growth-associated protein-43 modification of bone marrow mesenchymal stem cells improves traumatic optic neuropathy in rats	PubMed ID 26238991
Huang H, Lin F, Jiang J, Chen Y, Mei A, Zhu P. Stem Cell Res Ther. 2017 Oct 10;8(1):223. doi: 10.1186/s13287-017-0671-6. Effects of intra-arterial transplantation of adipose-derived stem cells on the expression of netrin-1 and its receptor DCC in ...	PubMed ID 29017609
Liu H, Lv P, Zhu Y, Wu H, Zhang K, Xu F, Zheng L, Zhao J. Sci Rep. 2017 Jan 5;7:39869. doi: 10.1038/srep39869. Salidroside promotes peripheral nerve regeneration based on tissue engineering strategy using Schwann cells and PLGA: in vitro and in vivo	PubMed ID 28054637
Zheng M, Chen R, Chen H, Zhang Y, Chen J, Lin P, Lan Q, Yuan Q, Lai Y, Jiang X, Pan X, Liu N. Front Cell Neurosci. 2018 Feb 13;12:13. doi: 10.3389/fncel.2018.00013. eCollection 2018. Netrin-1 Promotes Synaptic Formation and Axonal Regeneration via...	PubMed ID 29487502

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Customer Q&As

Questions and answers from customers related to MA1071 Anti-NEFH Antibody (Monoclonal, N52)

5 Related Questions

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-NEFH antibody (Monoclonal, N52) MA1071. Let me know if you need anything else.

Verified Customer

Asked: 2019-10-03

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-03

Question

We are currently using anti-NEFH antibody (Monoclonal, N52) MA1071 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?

P. Carter

Asked: 2018-01-19

Answer

The anti-NEFH antibody (Monoclonal, N52) (MA1071) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-01-19

Question

Does anti-NEFH antibody (Monoclonal, N52) MA1071 work on zebrafish IHC with brain?

Z. Li

Asked: 2017-08-31

Answer

Our lab technicians have not tested anti-NEFH antibody (Monoclonal, N52) MA1071 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-NEFH antibody (Monoclonal, N52) MA1071 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish brain in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-08-31

Question

How does your MA1071 NEFH antibody differ from SIGMA (ex-Millipore) Mouse Anti-Neurofilament 200 Kda Monoclonal antibody cat. no. MAB5262 clone: RT97? We intend to use it for immunohistochemistry on Fromalin Fixed tissues and paraffin embedded tissues.

Asked: 2019-11-06

Answer

The main difference would be the immunogen. Anti-NEFH antibody (Monoclonal, N52) MA1071 was raised against C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200. (uniprot: P16884). While the Mouse Anti-Neurofilament 200 Kda Monoclonal antibody cat.no. MAB5262 clone: RT97 Immunogen is Rat brain protein preparation (uniprot: P12036). Our MA1071 antibody should work for mouse samples with IHC-P and IHC-F.

Boster Scientific Support

Answered: 2019-11-06

Question

Is MA1071 suitable for immunostaining of mouse long bone nerve fibers?

Asked: 2019-10-06

Answer

Yes, the Anti-NEFH Antibody (Monoclonal, N52) (MA1071) is suitable for the immunostaining of mouse long bone nerve fibers.

Boster Scientific Support

Answered: 2019-10-09

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Domestically in USA and Canada, ships next business day if available in stock.

Note: sample size takes longer to process, average 5-7 days.

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Nefh Infographic

Comprehensive info on Nefh, beautifully presented.

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MA1071

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)

$240.00

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Anti-NEFH Antibody (Monoclonal, N52)

Anti-NEFH Antibody (Monoclonal, N52) Product Summary

Overview

Product Specs

Gene/Protein Basic Information For Nefh (Source: Uniprot.org, NCBI)

For more info on Nefh, check out the Nefh Infographic

We have 30,000+ of these available, one for each gene! Check them out.

Specific Protocols

Did You Know ?

Other Recommended Resources

Total number of citations: 12

0 reviews

Questions and answers from customers related to MA1071 Anti-NEFH Antibody (Monoclonal, N52)

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