| SKU: | MA1071 |
|---|---|
| Pack Size: | 100μg/vial |
| Validated Species: | Human, Mouse, Rat |
| Clonality: | Monoclonal |
| Clone Number: | N52 |
| Host: | Mouse |
| Application: | WB |
- Technical
Specs - Kit
Components - Gene
Information - Application
Data & Images - Reviews
Publications - Customer
Q&As
Overview
| Product Name | Anti-NEFH Antibody (Monoclonal, N52) |
|---|---|
| SKU/Catalog Number | MA1071 |
| Description | Mouse IgG monoclonal antibody for NEFH, neurofilament, heavy polypeptide (NEFH) detection. Tested with WB, IHC-P, IHC-F in Human;mouse;rat. No cross reactivity with other proteins. |
| Cite This Product | Anti-NEFH Antibody (Monoclonal, N52) N52 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1071) |
| Replacement Item | This antibody may replace the following items: sc-133165|sc-133237|sc-137009|sc-20014|sc-20015|sc-20112|sc-22909|sc-32273|sc-32728|sc-32729|sc-32730|sc-51682|sc-52350|sc-53798|sc-56575|sc-58553 from Santa Cruz Biotechnology. |
| Host | Mouse |
| Validated Species | Human, Mouse, Rat |
| Application | WB *Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under Protein Target Info. |
| Recommended Detection Systems | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $50. Contact us for more information **Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018! |
| Immunogen | C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200. |
| Cross Reactivity | No cross reactivity with other proteins |
| Pack Size | 100μg/vial |
Properties
| Clonality | Monoclonal |
|---|---|
| Clone Number | N52 |
| Form | Lyophilized |
| Contents | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Concentration | Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
| Storage | At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing. |
| Purification | Ascites |
| Isotype | Mouse IgG1 |
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
| Gene Name | Nefh |
|---|---|
| Protein Name | Neurofilament heavy polypeptide |
| Molecular Weight | 115378 MW |
| Protein Function | Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. |
| Sequence Similarities | Belongs to the intermediate filament family. |
| Uniprot ID | P16884 |
| Alternative Names | Neurofilament heavy polypeptide;NF-H;200 kDa neurofilament protein;Neurofilament triplet H protein;Nefh;Nfh; |
| Research Areas | |neuroscience|neurology process|neurodegenerative disease|alzheimer's disease| neuroscience|cell adhesion proteins|cytoskeletal proteins|intermediate filaments| signal transduction|cytoskeleton / ecm|cytoskeleton|class iv|neurofilaments|cell type marker|neuron marker|axon marker| |
Background for Neurofilament heavy polypeptide
Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 kD(NFL), 125 kD(NFM), and 200 kD(NFH) on SDS-gel electrophoresis. Genomic clones for the largest human neurofilament protein(NF-H) were isolated, the intron/exon boundaries mapped and the entire protein-coding regions(exons) sequenced. mutations in neurofilaments have been linked to some forms of Charcot-Marie-Tooth disease(CMT).
Related Products
Goat Anti-Mouse IgG Secondary Antibody, HRP Conjugate (BA1054)
Price: FREE if purchase with Primary antibody
Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001)
Price: $100
SuperVision IHC Kit with anti-Mouse IgG (SV0001-1)
Price: $150
Anti-NEFH Antibody (Monoclonal, N52) Images
Click the images to enlarge.
Anti-NF200 antibody (monoclonal), MA1071, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
Publications
Zheng M, Chen R, Chen H, Zhang Y, Chen J, Lin P, Lan Q, Yuan Q, Lai Y, Jiang X, Pan X, Liu N. Front Cell Neurosci. 2018 Feb 13;12:13. doi: 10.3389/fncel.2018.00013. eCollection 2018. Netrin-1 Promotes Synaptic Formation and Axonal Regeneration via JNK1/c-Jun Pathway after the Middle Cerebral Artery Occlusion
Customer Q&As
Q: Is your western blot protocol provided from the website applicable for all your antibodies?
Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody?
Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to nf200 antibody, nf-h antibody
