Anti-NEFH Antibody (Monoclonal, N52)

Boster Bio Anti-NEFH Antibody (Monoclonal, N52) catalog # MA1071. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 12 publication(s).

Product Info Summary

SKU: MA1071
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: IHC, WB

Product Name

Anti-NEFH Antibody (Monoclonal, N52)

See all NF-H products

SKU/Catalog Number

MA1071

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-NEFH Antibody (Monoclonal, N52) catalog # MA1071. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NEFH Antibody (Monoclonal, N52) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1071)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

N52

Isotype

Mouse IgG1

Immunogen

C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1071 is reactive to Nefh in Human, Mouse, Rat

Applications

MA1071 is guaranteed for IHC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, By Heat
Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, mouse, rat, -
Western blot, 0.5ml, Human, mouse, rat

Validation Images & Assay Conditions

Gene/Protein Information For Nefh (Source: Uniprot.Org, NCBI)

Uniprot ID

P16884

Gene ID

24587

Gene Name

Nefh

Full Name

Neurofilament heavy polypeptide

Weight

115.378kDa

Superfamily

intermediate filament family

Alternative Names

200 kDa neurofilament protein; KIAA0845; NEFH; Neurofilament Heavy (200kDa); neurofilament heavy polypeptide; Neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa; neurofilament, heavy polypeptide; NF200; NFH; NF-H Nefh|Nfh|neurofilament heavy|neurofilament heavy polypeptide|200 kDa neurofilament protein|NF-H|neurofilament triplet H protein|neurofilament, heavy polypeptide

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Nefh, check out the Nefh Infographic

Nefh infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Nefh: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

MA1071 has been cited in 12 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Wu K,Yue J,Shen K,He J,Zhu G,Liu S,Zhang C,Yang H. Increased Expression of Fibroblast Growth Factor 13 in Cortical Lesions of the Focal Cortical Dysplasia. Brain Res Bull. 2020 Dec 4:S0361-9230(20)30710-3.doi: 10.1016/j.brainresbull.2020.11.023.Epub ahead
Species: Human
MA1071 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:50

Changes in the Expression of miR-34a and its Target Genes Following Spinal Cord Injury In Rats

MicroRNA-30b regulates expression of the sodium channel Nav17 in nerve injury-induced neuropathic pain in the rat

Neuron-derived IgG Protects Neurons from Complement-dependent Cytotoxicity

An Acetylcholinesterase Antibody-Based Quartz Crystal Microbalance for the Rapid Identification of Spinal Ventral and Dorsal Roots

Trimebutine, a small molecule mimetic agonist of adhesion molecule L1, contributes to functional recovery after spinal cord injury in mice

Expression of pannexin 1 and 2 in cortical lesions from intractable epilepsy patients with focal cortical dysplasia

Downregulation of CD47 and CD200 in patients with focal cortical dysplasia type IIb and tuberous sclerosis complex

Lentiviral-mediated growth-associated protein-43 modification of bone marrow mesenchymal stem cells improves traumatic optic neuropathy in rats

Huang H, Lin F, Jiang J, Chen Y, Mei A, Zhu P. Stem Cell Res Ther. 2017 Oct 10;8(1):223. doi: 10.1186/s13287-017-0671-6. Effects of intra-arterial transplantation of adipose-derived stem cells on the expression of netrin-1 and its receptor DCC in ...

Liu H, Lv P, Zhu Y, Wu H, Zhang K, Xu F, Zheng L, Zhao J. Sci Rep. 2017 Jan 5;7:39869. doi: 10.1038/srep39869. Salidroside promotes peripheral nerve regeneration based on tissue engineering strategy using Schwann cells and PLGA: in vitro and in vivo

Zheng M, Chen R, Chen H, Zhang Y, Chen J, Lin P, Lan Q, Yuan Q, Lai Y, Jiang X, Pan X, Liu N. Front Cell Neurosci. 2018 Feb 13;12:13. doi: 10.3389/fncel.2018.00013. eCollection 2018. Netrin-1 Promotes Synaptic Formation and Axonal Regeneration via...

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5 Customer Q&As for Anti-NEFH Antibody (Monoclonal, N52)

Question

How does your MA1071 NEFH antibody differ from SIGMA (ex-Millipore) Mouse Anti-Neurofilament 200 Kda Monoclonal antibody cat. no. MAB5262 clone: RT97? We intend to use it for immunohistochemistry on Fromalin Fixed tissues and paraffin embedded tissues.

Verified customer

Asked: 2019-11-06

Answer

The main difference would be the immunogen. Anti-NEFH antibody (Monoclonal, N52) MA1071 was raised against C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200. (uniprot: P16884). While the Mouse Anti-Neurofilament 200 Kda Monoclonal antibody cat.no. MAB5262 clone: RT97 Immunogen is Rat brain protein preparation (uniprot: P12036). Our MA1071 antibody should work for mouse samples with IHC-P and IHC-F.

Boster Scientific Support

Answered: 2019-11-06

Question

Is MA1071 suitable for immunostaining of mouse long bone nerve fibers?

Verified customer

Asked: 2019-10-06

Answer

Yes, the Anti-NEFH Antibody (Monoclonal, N52) (MA1071) is suitable for the immunostaining of mouse long bone nerve fibers.

Boster Scientific Support

Answered: 2019-10-09

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-NEFH antibody (Monoclonal, N52) MA1071. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-10-03

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-03

Question

We are currently using anti-NEFH antibody (Monoclonal, N52) MA1071 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?

P. Carter

Verified customer

Asked: 2018-01-19

Answer

The anti-NEFH antibody (Monoclonal, N52) (MA1071) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-01-19

Question

Does anti-NEFH antibody (Monoclonal, N52) MA1071 work on zebrafish IHC with brain?

Z. Li

Verified customer

Asked: 2017-08-31

Answer

Our lab technicians have not tested anti-NEFH antibody (Monoclonal, N52) MA1071 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-NEFH antibody (Monoclonal, N52) MA1071 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish brain in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-08-31

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