Anti-Nefh Antibody Picoband™

NF-H antibody

Boster Bio Anti-Nefh Antibody Picoband™ catalog # A05307. Tested in ELISA, Flow Cytometry, IHC-P, WB applications. This antibody reacts with Mouse, Rat. Cited in 17 publication(s).

Product Info Summary

SKU: A05307
Size: 100μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IHC-P, WB

Product Name

Anti-Nefh Antibody Picoband™

View all NF-H Antibodies

SKU/Catalog Number

A05307

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Nefh Antibody Picoband™ catalog # A05307. Tested in ELISA, Flow Cytometry, IHC-P, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Nefh Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05307)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Polyclonal

Immunogen

E.coli-derived mouse Nefh recombinant protein (Position: Y109-E466).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05307 is reactive to Nefh in Mouse, Rat

Applications

A05307 is guaranteed for ELISA, Flow Cytometry, IHC-P, WB Boster Guarantee

Background of NF-H

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat
Flow Cytometry, 1-3μg/1x106 cells, Mouse
Direct ELISA, 0.1-0.5μg/ml, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For Nefh (Source: Uniprot.Org, NCBI)

Uniprot ID

P19246

Gene ID

380684

Gene Name

Nefh

Full Name

Neurofilament heavy polypeptide

Weight

116.994kDa

Superfamily

intermediate filament family

Alternative Names

200 kDa neurofilament protein; KIAA0845; NEFH; Neurofilament Heavy (200kDa); neurofilament heavy polypeptide; Neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa; neurofilament, heavy polypeptide; NF200; NFH; NF-H Nefh|NEF, NF-, NF-H, NF20, NF200, Nfh, mKIAA0845|neurofilament, heavy polypeptide|neurofilament heavy polypeptide|200 kDa neurofilament protein|neurofilament 200kDa|neurofilament triplet H protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Nefh, check out the Nefh Infographic

Nefh infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Nefh: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A05307 has been cited in 17 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Gene silencing NMII promotes axonal regeneration against contusive spinal cord injury in rats

Bone Marrow Mesenchymal Stem Cells and Electroacupuncture Downregulate the Inhibitor Molecules and Promote the Axonal Regeneration in the Transected Spinal Cord of Rats:

Improved Retinal Ganglion Cell Survival through Retinal Microglia Suppression by a Chinese Herb Extract, Triptolide, in the DBA/2J Mouse Model of Glaucoma

Apolipoprotein E 4 triggers multiple pathway-mediated Ca2+ overload, causes CaMK II phosphorylation abnormity and aggravates oxidative stress caused cerebral cortical neuron damage

Changes in the Expression of miR-34a and its Target Genes Following Spinal Cord Injury In Rats

Downregulation of CD47 and CD200 in patients with focal cortical dysplasia type IIb and tuberous sclerosis complex

Increased Expression and Cellular Localization of P2X7R in Cortical Lesions of Patients With Focal Cortical Dysplasia

Vaccination with dendritic cells pulsed with homogenate protein of spinal cord promotes functional recovery from spinal cord injury in mice

Biocompatible Chitin Hydrogel Incorporated with PEDOT Nanoparticles for Peripheral Nerve Repair

Human mesenchymal stem cells improve the neurodegeneration of femoral nerve in a diabetic foot ulceration rats

Amelioration of β-amyloid-induced cognitive dysfunction and hippocampal axon degeneration by curcumin is associated with suppression of CRMP-2 hyperphosphorylation

Implantation of adult bone marrow-derived mesenchymal stem cells transfected with the neurotrophin-3 gene and pretreated with retinoic acid in completely transected spinal cord

Endogenous Subventricular Zone Neural Progenitors Contribute to the Formation and Hyperexcitability of Experimental Model of Focal Microgyria

Altered expression of neurofilament 200 and amyloid-β peptide (1–40) in a rat model of chronic cerebral hypoperfusion

Cograft of neural stem cells and schwann cells overexpressing TrkC and neurotrophin-3 respectively after rat spinal cord transection
Species: Rat

Zhang Ym, Zhang Yq, Cheng Sb, Chen Sx, Chen Al, Tang Cz. Chin J Integr Med. 2013 Feb;19(2):132-6. Doi: 10.1007/S11655-013-1353-6. Epub 2013 Jan 31. Effect Of Acupuncture On Proliferation And Differentiation Of Neural Stem Cells In Brain Tissues Of...

He Jj, Li S, Shu Hf, Yu Sx, Liu Sy, Yin Q, Yang H. J Neuropathol Exp Neurol. 2013 Feb;72(2):152-63. Doi: 10.1097/Nen.0B013E318281262E. The Interleukin 17 System In Cortical Lesions In Focal Cortical Dysplasias.

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Total: $315

Size:100μg

SKU:A05307

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A05307
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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