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Product Info Summary
| SKU: | A06730-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-NEGR1 Antibody Picoband™
View all Kilon/NEGR1 Antibodies
SKU/Catalog Number
A06730-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-NEGR1 Antibody Picoband™ catalog # A06730-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NEGR1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06730-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NEGR1 recombinant protein (Position: Q36-H291).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06730-2 is reactive to Negr1 in Human, Mouse, Rat
Applications
A06730-2 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
46 kDa
Calculated molecular weight
37.858kDa
Background of Kilon/NEGR1
Neuronal growth regulator 1 also known as NEGR1 is a protein which in humans is encoded by the NEGR1 gene. NEGR1 may be involved in cell-adhesion. May function as a trans-neural growth-promoting factor in regenerative axon sprouting in the mammalian brain (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Mouse, Rat
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NEGR1 using anti-NEGR1 antibody (A06730-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEGR1 antigen affinity purified polyclonal antibody (Catalog # A06730-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEGR1 at approximately 46 kDa. The expected band size for NEGR1 is at 39 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Negr1 (Source: Uniprot.org, NCBI)
Gene Name
Negr1
Full Name
Neuronal growth regulator 1
Weight
37.858kDa
Superfamily
immunoglobulin superfamily
Alternative Names
a kindred of IgLON; IgLON family member 4; IGLON4DMML2433; Kilon; KILONMGC46680; NEGR1; neuronal growth regulator 1; Neurotractin; Ntra Negr1|neuronal growth regulator 1|neuronal growth regulator 1|kilon protein|kindred of IgLON
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Negr1, check out the Negr1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Negr1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NEGR1 Antibody Picoband™ (A06730-2)
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No publications found for A06730-2
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