Product Info Summary
SKU: | M01991 |
---|---|
Size: | 100 ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IP, WB |
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Product info
Product Name
Anti-nephrin Antibody
SKU/Catalog Number
M01991
Size
100 ul
Description
Anti-nephrin Antibody
Storage & Handling
Storage: - 20 °C. Shipping Condition: +2 oC to +8 °C
Cite This Product
Anti-nephrin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01991)
Host
Rabbit
Contents
Buffer: 20 mM Tris-HCl, pH 8.0. Stanilizer: 10 mg/ml BSA
Clonality
Monoclonal
Clone Number
Y17-R
Isotype
Rabbit IgG
Immunogen
Peptide derived from the centre of the fifth Ig-like domain of human nephrin. Antibody recognizes the epitope located between Cys465 - Cys528.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01991 is reactive to NPHS1 in Human, Mouse, Rat
Applications
M01991 is guaranteed for ELISA, IP, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
134.742kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting – 1:5,000
ELISA – 1:50,000-1:100,000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of mouse nephrin: lane 1 – 50 ng; lane 2 – 100 ng; lane 3 – 200 ng of recombinant mouse nephrin
Click image to see more details
Representative picture of Nephrin expression in HEK293 cells
Protein Target Info & Infographic
Gene/Protein Information For NPHS1 (Source: Uniprot.org, NCBI)
Gene Name
NPHS1
Full Name
Nephrin
Weight
134.742kDa
Superfamily
immunoglobulin superfamily
Alternative Names
CNF; Nephrin; nephrosis 1, congenital, Finnish type (nephrin); NPHNCNF; NPHS1; Renal glomerulus-specific cell adhesion receptor NPHS1 CNF, NPHN, nephrin NPHS1 adhesion molecule, nephrin nephrin|NPHS1, nephrin|nephrosis 1, congenital, Finnish type (nephrin)|renal glomerulus-specific cell adhesion receptor|truncated NPHS1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NPHS1, check out the NPHS1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NPHS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-nephrin Antibody (M01991)
Hello CJ!
M01991 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Qiu Y,Zhou J,Zhang H,Zhou H,Tang H,Lei C,Ye C,You C,Chen Y,Wang Y,Xiong J,Su H,Yao G,Zhang C.Rhodojaponin II attenuates kidney injury by regulating TGF-β1/Smad pathway in mice with adriamycin nephropathy.J Ethnopharmacol.2019 Oct 28;243:112078.doi:10.1016/j.jep.2019.112078.Epub 2019 Jul 10.PMID: 31301369.
Species: Mouse
M01991 usage in article: APP:WB, SAMPLE:RENAL TISSUE, DILUTION:1:200
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