Anti-Nestin Monoclonal Antibody

Boster Bio Anti-Nestin Monoclonal Antibody catalog # M00806-1. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 18 publication(s).

Product Info Summary

SKU: M00806-1
Size: 100ul
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: IF, IHC, ICC, WB

Product Name

Anti-Nestin Monoclonal Antibody

See all Nestin products

SKU/Catalog Number

M00806-1

Size

100ul

Description

Boster Bio Anti-Nestin Monoclonal Antibody catalog # M00806-1. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Nestin Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00806-1)

Host

Mouse

Contents

Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3

Clonality

Monoclonal

Immunogen

Recombinant construct, amino acids 317-630 of the human protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00806-1 is reactive to NES in Human, Mouse, Rat

Applications

M00806-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB: 1:5,000 IF/ICC: 1:500.

Validation Images & Assay Conditions

Gene/Protein Information For NES (Source: Uniprot.Org, NCBI)

Uniprot ID

P48681

Gene ID

10763

Gene Name

NES

Full Name

Nestin

Weight

177.439kDa

Superfamily

intermediate filament family

Alternative Names

FLJ21841; NES; Nestin NES Nbla00170 nestin nestin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on NES, check out the NES Infographic

NES infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for NES: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M00806-1 has been cited in 18 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Meng FW,Liu FS,Liu WH,Li L,Jie LL.Formation of new lymphatic vessels in glioma: An immunohistochemical analysis.Neuropathology.2020 Jun;40(3):215-223.doi:10.1111/neup.12625.Epub 2020 Jan 20.PMID:31960509;PMCID:PMC 7317190.
Species: Human
M00806-1 usage in article: APP:IHC, SAMPLE:GILOMA TISSUE, DILUTION:1:100

Isolation of neural crest%u2010derived stem cells from rat embryonic mandibular processes

Expression of nestin in the podocytes of normal and diseased human kidneys

Molecular and immunochemical demonstration of a novel member of Bf/C2 homolog in amphioxus Branchiostoma belcheri: implications for involvement of hepatic %u2026

Distribution and localization of fibroblast growth factor-8 in rat brain and nerve cells during neural stem/progenitor cell differentiation

Ipsilateral versus bilateral limb-training in promoting the proliferation and differentiation of endogenous neural stem cells following cerebral infarction in rats

Impaired Spatial Learning and Memory after Sevoflurane?Nitrous Oxide Anesthesia in Aged Rats Is Associated with Down-Regulated cAMP/CREB Signaling

Insulin-Producing Cells Derived from Human Embryonic Stem Cells: Comparison of Definitive Endoderm- and Nestin-Positive Progenitor-Based Differentiation Strategies

Differentiation of human bone marrow-derived mesenchymal stem cells into neural-like cells by co-culture with retinal pigmented epithelial cells

Regulated differentiation of WERI-Rb-1 cells into retinal neuron-like cells

Effect of Corilagin on the Proliferation and NF-?B in U251 Glioblastoma Cells and U251 Glioblastoma Stem-Like Cells

Propofol and remifentanil at moderate and high concentrations affect proliferation and differentiation of neural stem/progenitor cells

Characterization and retinal neuron differentiation of WERI-Rb1 cancer stem cells

Luo L, Deng S, Yi J, Zhou S, She Y, Liu B. Evid Based Complement Alternat Med. 2017;2017:4072658. doi: 10.1155/2017/4072658. Epub 2017 Mar 8. Buyang Huanwu Decoction Ameliorates Poststroke Depression via Promoting Neurotrophic Pathway Mediated Neu...

Li M, Xie ZH, Yu AY, He DP. Chin Med J (Engl). 2018 Feb 5;131(3):323-329. doi: 10.4103/0366-6999.223840. Increased Efficacy of Antivenom Combined with Hyperbaric Oxygen on Deinagkistrodon acutus Envenomation in Adult Rats

Bin Wt, Ma Lm, Xu Q, Shi Xl. World J Gastroenterol. 2012 Jan 28;18(4):309-22. Doi: 10.3748/Wjg.V18.I4.309. Embryonic Hepatocyte Transplantation For Hepatic Cirrhosis: Efficacy And Mechanism Of Action.

Liu Q, Cheng G, Wang Z, Zhan S, Xiong B, Zhao X. In Vitro Cell Dev Biol Anim. 2015 Mar;51(3):319-27. Doi: 10.1007/S11626-015-9875-1. Epub 2015 Mar 14. Bone Marrow-Derived Mesenchymal Stem Cells Differentiate Into Nerve-Like Cells In Vitro After Tr...

Xu Yl, Sun Jg, Wu Fb, Xi Ym. J Stem Cells Regen Med. 2011 Oct 30;7(2):69-74. Ecollection 2011. Investigation Of Characteristics Of Feather Follicle Stem Cells And Their Regeneration Potential.

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16 Customer Q&As for Anti-Nestin Monoclonal Antibody

Question

See attached the WB image, lot number and protocol we used for placenta using anti-Nestin Monoclonal antibody M00806-1. Please let me know if you require anything else.

J. Anderson

Verified customer

Asked: 2020-03-25

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-25

Question

Is this M00806-1 anti-Nestin Monoclonal antibody reactive to the isotypes of NES?

Verified Customer

Verified customer

Asked: 2020-02-17

Answer

The immunogen of M00806-1 anti-Nestin Monoclonal antibody is Recombinant construct, amino acids 317-630 of the human protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-02-17

Question

I was wanting to use to test anti-Nestin Monoclonal antibody M00806-1 on mouse placenta for research purposes, then I may be interested in using anti-Nestin Monoclonal antibody M00806-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-01-28

Answer

The products we sell, including anti-Nestin Monoclonal antibody M00806-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-01-28

Question

We have been able to see staining in rat cervix carcinoma. What should we do? Is anti-Nestin Monoclonal antibody supposed to stain cervix carcinoma positively?

Verified Customer

Verified customer

Asked: 2019-12-31

Answer

According to literature cervix carcinoma does express NES. According to Uniprot.org, NES is expressed in metanephric glomerulus, placenta, neuroblastoma, cervix carcinoma, liver, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have NES expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 18318008, 24275569
Neuroblastoma, Pubmed ID: 12880961
Placenta, Pubmed ID: 1478958, 16710414

Boster Scientific Support

Answered: 2019-12-31

Question

Does M00806-1 anti-Nestin Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-12-19

Answer

As indicated on the product datasheet, M00806-1 anti-Nestin Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-12-19

Question

We ordered your anti-Nestin Monoclonal antibody for WB on metanephric glomerulus a few years ago. I am using mouse, and We want to use the antibody for IHC next. you antibody examining metanephric glomerulus as well as neuroblastoma in our next experiment. Could give a recommendation on which antibody would work the best for IHC?

M. Roberts

Verified customer

Asked: 2019-10-11

Answer

I took a look at the website and datasheets of our anti-Nestin Monoclonal antibody and it appears that M00806-1 has been validated on mouse in both WB and IHC. Thus M00806-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-10-11

Question

Would anti-Nestin Monoclonal antibody M00806-1 work on zebrafish WB with metanephric glomerulus?

Verified Customer

Verified customer

Asked: 2019-10-11

Answer

Our lab technicians have not validated anti-Nestin Monoclonal antibody M00806-1 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Nestin Monoclonal antibody M00806-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish metanephric glomerulus in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-11

Question

I have a question about product M00806-1, anti-Nestin Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-08-06

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00806-1 anti-Nestin Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-08-06

Question

We are currently using anti-Nestin Monoclonal antibody M00806-1 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-15

Answer

The anti-Nestin Monoclonal antibody (M00806-1) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-15

Question

Does anti-Nestin Monoclonal antibody M00806-1 work for IHC with placenta?

Verified Customer

Verified customer

Asked: 2019-06-20

Answer

According to the expression profile of placenta, NES is highly expressed in placenta. So, it is likely that anti-Nestin Monoclonal antibody M00806-1 will work for IHC with placenta.

Boster Scientific Support

Answered: 2019-06-20

Question

Is a blocking peptide available for product anti-Nestin Monoclonal antibody (M00806-1)?

Verified Customer

Verified customer

Asked: 2019-06-07

Answer

We do provide the blocking peptide for product anti-Nestin Monoclonal antibody (M00806-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-06-07

Question

I see that the anti-Nestin Monoclonal antibody M00806-1 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-07-02

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-07-02

Question

Is there a BSA free version of anti-Nestin Monoclonal antibody M00806-1 available?

Verified Customer

Verified customer

Asked: 2017-12-13

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Nestin Monoclonal antibody M00806-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-12-13

Question

I was wanting to use your anti-Nestin Monoclonal antibody for IHC for mouse placenta on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse placenta identification?

Verified Customer

Verified customer

Asked: 2017-10-25

Answer

It shows on the product datasheet, M00806-1 anti-Nestin Monoclonal antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse placenta in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-10-25

Question

I am interested in using your anti-Nestin Monoclonal antibody for negative regulation of catalytic activity studies. Has this antibody been tested with western blotting on human neuroblastoma cells? We would like to see some validation images before ordering.

J. Collins

Verified customer

Asked: 2016-09-27

Answer

We appreciate your inquiry. This M00806-1 anti-Nestin Monoclonal antibody is validated on rat glioma cells, human neuroblastoma cells. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2016-09-27

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-Nestin Monoclonal antibody M00806-1. Let me know if you need anything else.

L. Walker

Verified customer

Asked: 2015-02-17

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-02-17

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M00806-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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