Anti-Nestin Rabbit Monoclonal Antibody

Boster Bio Anti-Nestin Rabbit Monoclonal Antibody catalog # M00806. Tested in IHC applications. This antibody reacts with Human. Cited in 13 publication(s).

Product Info Summary

SKU: M00806
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: IF, IHC, ICC

Product Name

Anti-Nestin Rabbit Monoclonal Antibody

See all Nestin products

SKU/Catalog Number

M00806

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Nestin Rabbit Monoclonal Antibody catalog # M00806. Tested in IF, IHC, ICC applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Nestin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00806)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

FBH-14

Immunogen

A synthesized peptide derived from human Nestin

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00806 is reactive to NES in Human

Applications

M00806 is guaranteed for IF, IHC, ICC Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

IHC 1:50-1:200
ICC/IF 1:50-1:200

Validation Images & Assay Conditions

Gene/Protein Information For NES (Source: Uniprot.Org, NCBI)

Uniprot ID

P48681

Gene ID

10763

Gene Name

NES

Full Name

Nestin

Weight

177.439kDa

Superfamily

intermediate filament family

Alternative Names

FLJ21841; NES; Nestin NES Nbla00170 nestin nestin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on NES, check out the NES Infographic

NES infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for NES: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M00806 has been cited in 13 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Meng FW,Liu FS,Liu WH,Li L,Jie LL.Formation of new lymphatic vessels in glioma: An immunohistochemical analysis.Neuropathology.2020 Jun;40(3):215-223.doi:10.1111/neup.12625.Epub 2020 Jan 20.PMID:31960509;PMCID:PMC 7317190.
Species: Human
M00806 usage in article: APP:IHC, SAMPLE:GILOMA TISSUE, DILUTION:1:100

Isolation of neural crest%u2010derived stem cells from rat embryonic mandibular processes

Expression of nestin in the podocytes of normal and diseased human kidneys

Molecular and immunochemical demonstration of a novel member of Bf/C2 homolog in amphioxus Branchiostoma belcheri: implications for involvement of hepatic %u2026

Ipsilateral versus bilateral limb-training in promoting the proliferation and differentiation of endogenous neural stem cells following cerebral infarction in rats

Insulin-Producing Cells Derived from Human Embryonic Stem Cells: Comparison of Definitive Endoderm- and Nestin-Positive Progenitor-Based Differentiation Strategies

Differentiation of human bone marrow-derived mesenchymal stem cells into neural-like cells by co-culture with retinal pigmented epithelial cells

Regulated differentiation of WERI-Rb-1 cells into retinal neuron-like cells

Effect of Corilagin on the Proliferation and NF-?B in U251 Glioblastoma Cells and U251 Glioblastoma Stem-Like Cells

Effect of siRNA-Livin on drug resistance to chemotherapy in glioma U251 cells and CD133+ stem cells

Characterization and retinal neuron differentiation of WERI-Rb1 cancer stem cells

Liu Q, Cheng G, Wang Z, Zhan S, Xiong B, Zhao X. In Vitro Cell Dev Biol Anim. 2015 Mar;51(3):319-27. Doi: 10.1007/S11626-015-9875-1. Epub 2015 Mar 14. Bone Marrow-Derived Mesenchymal Stem Cells Differentiate Into Nerve-Like Cells In Vitro After Tr...

Xu Yl, Sun Jg, Wu Fb, Xi Ym. J Stem Cells Regen Med. 2011 Oct 30;7(2):69-74. Ecollection 2011. Investigation Of Characteristics Of Feather Follicle Stem Cells And Their Regeneration Potential.

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3 Customer Q&As for Anti-Nestin Rabbit Monoclonal Antibody

Question

We have observed staining in human liver. What should we do? Is anti-Nestin Rabbit Monoclonal antibody supposed to stain liver positively?

Verified Customer

Verified customer

Asked: 2020-04-30

Answer

According to literature liver does express NES. According to Uniprot.org, NES is expressed in metanephric glomerulus, placenta, neuroblastoma, cervix carcinoma, liver, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have NES expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 18318008, 24275569
Neuroblastoma, Pubmed ID: 12880961
Placenta, Pubmed ID: 1478958, 16710414

Boster Scientific Support

Answered: 2020-04-30

Question

I am interested in using your anti-Nestin Rabbit Monoclonal antibody for negative regulation of protein binding studies. Has this antibody been tested with western blotting on human melanoma tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-01-29

Answer

We appreciate your inquiry. This M00806 anti-Nestin Rabbit Monoclonal antibody is tested on human melanoma tissue. It is guaranteed to work for IHC in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-01-29

Question

We are currently using anti-Nestin Rabbit Monoclonal antibody M00806 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?

A. Williams

Verified customer

Asked: 2016-03-09

Answer

The anti-Nestin Rabbit Monoclonal antibody (M00806) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-03-09

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
M00806
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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