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- Table of Contents
NES antibody (M00806) performs well in IF, IHC, WB and shows reactivity with Human, Mouse, Pig, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 8 Publications
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IF analysis of Nestin using anti-Nestin antibody (M00806).
Nestin was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with rabbit anti-Nestin Antibody (M00806) at a dilution of 1:50 overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Nestin was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with rabbit anti-Nestin Antibody (M00806) at a dilution of 1:50 overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-Nestin Rabbit Monoclonal Antibody
NES antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Pig, Rat | Rabbit | IF, IHC, WB |
Tested Applications
NES antibody was positively detected by WB in:
human U251 whole cell, human SH-SY5Y whole cell, rat C6 whole cell, mouse Neuro-2a whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
NES antibody was positively detected by IHC in:
human diffuse large B-cell lymphoma of the intestine tissue, human glioma tissue, human non-small cell lung cancer tissue, mouse brain tissue, rat brain tissue, pig brain tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
NES antibody was positively detected by IF in:
human brain tissue, rat brain tissue
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
IF 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Tested
Rat
Tested
Tested
Tested
Mouse
Tested
Tested
Recommend
Pig
Tested
Recommend
Product Information
Product Name
Anti-Nestin Rabbit Monoclonal Antibody
View all NES Antibodies
View all NES Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FBH-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Nestin
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
270-300 kDa
Calculated Molecular Weight
177.4 kDa
Cite This Product
Anti-Nestin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00806)
Protocols
Western Blot protocol for NES antibody
Immunohistochemistry (IHC) protocol for NES antibody
Immunofluorescence (IF) protocol for NES antibody
Publications
Application:
Species:
LPS‑mediated adaptation accelerates ecto‑MSCs differentiation into osteoblasts
Published: 2024-10-15
Journal: Molecular Medicine Reports
Application: IF
Impact Factor: 3.40
Species: Human
PMID: 39422037
Improved Cell Properties of Human Dental Pulp Stem Cells (hDPSCs) Isolated and Expanded in a GMP Compliant and Xenogeneic Serum-free Medium
Published: 2023-11-01
Journal: IN VIVO
Application: IF
Impact Factor: 2.30
Species: Human
PMID: 37905631
Electrical stimulation induced structural 3D human engineered neural tissue with well-developed neuronal network and functional connectivity
Published: 2023-07-20
Journal: Journal of Neural Engineering
Application: IF
Impact Factor: 4.00
Species: Human
PMID: 37433290
Global Transcriptional Analyses of the Wnt-Induced Development of Neural Stem Cells from Human Pluripotent Stem Cells
Published: 2021-07-12
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Application: ICC
Impact Factor: 5.92
Species: Human
PMID: 34299091
Formation of new lymphatic vessels in glioma: An immunohistochemical analysis
Published: 2020-01-20
Journal: NEUROPATHOLOGY
Application: IHC
Impact Factor: 1.76
Species: Human
PMID: 31960509
Microvesicles Derived from Human Embryonic Neural Stem Cells Inhibit the Apoptosis of HL-1 Cardiomyocytes by Promoting Autophagy and Regulating AKT and mTOR via Transporting HSP-70
Published: 2019-10-24
Journal: Stem Cells International
Application: IF
Impact Factor: 3.90
Species: Human
PMID: 31772588
Radiation-induced G2/M arrest rarely occurred in glioblastoma stem-like cells
Published: 2018-02-27
Journal: INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
Application: IF
Impact Factor: 1.97
Species: Human
PMID: 29463172
Insulin-Producing Cells Derived from Human Embryonic Stem Cells: Comparison of Definitive Endoderm- and Nestin-Positive Progenitor-Based Differentiation Strategies
Published: 2013-08-12
Journal: PLoS One
Application: IF
Impact Factor: 3.73
Species: Human
PMID: 23951327
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Product Promise
We promise Anti-Nestin Rabbit Monoclonal Antibody performs excellently in relevant applications for IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00806
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026