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Product Info Summary
|Application:||IF, IHC, ICC|
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Anti-Nestin Rabbit Monoclonal Antibody
Boster Bio Anti-Nestin Rabbit Monoclonal Antibody catalog # M00806. Tested in IF, IHC, ICC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Nestin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00806)
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
A synthesized peptide derived from human Nestin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
M00806 is reactive to NES in Human
M00806 is guaranteed for IF, IHC, ICC Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human melanoma tissue labeling Nestin(M00806)
NES was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NES Antibody (M00806)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?? The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Gene/Protein Information For NES (Source: Uniprot.Org, NCBI)
intermediate filament family
FLJ21841; NES; Nestin NES Nbla00170 nestin nestin*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on NES, check out the NES Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for NES: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
M00806 has been cited in 13 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Meng FW,Liu FS,Liu WH,Li L,Jie LL.Formation of new lymphatic vessels in glioma: An immunohistochemical analysis.Neuropathology.2020 Jun;40(3):215-223.doi:10.1111/neup.12625.Epub 2020 Jan 20.PMID:31960509;PMCID:PMC 7317190.
M00806 usage in article: APP:IHC, SAMPLE:GILOMA TISSUE, DILUTION:1:100
Isolation of neural crest%u2010derived stem cells from rat embryonic mandibular processes
Expression of nestin in the podocytes of normal and diseased human kidneys
Molecular and immunochemical demonstration of a novel member of Bf/C2 homolog in amphioxus Branchiostoma belcheri: implications for involvement of hepatic %u2026
Ipsilateral versus bilateral limb-training in promoting the proliferation and differentiation of endogenous neural stem cells following cerebral infarction in rats
Insulin-Producing Cells Derived from Human Embryonic Stem Cells: Comparison of Definitive Endoderm- and Nestin-Positive Progenitor-Based Differentiation Strategies
Differentiation of human bone marrow-derived mesenchymal stem cells into neural-like cells by co-culture with retinal pigmented epithelial cells
Regulated differentiation of WERI-Rb-1 cells into retinal neuron-like cells
Effect of Corilagin on the Proliferation and NF-?B in U251 Glioblastoma Cells and U251 Glioblastoma Stem-Like Cells
Effect of siRNA-Livin on drug resistance to chemotherapy in glioma U251 cells and CD133+ stem cells
Characterization and retinal neuron differentiation of WERI-Rb1 cancer stem cells
Liu Q, Cheng G, Wang Z, Zhan S, Xiong B, Zhao X. In Vitro Cell Dev Biol Anim. 2015 Mar;51(3):319-27. Doi: 10.1007/S11626-015-9875-1. Epub 2015 Mar 14. Bone Marrow-Derived Mesenchymal Stem Cells Differentiate Into Nerve-Like Cells In Vitro After Tr...
Xu Yl, Sun Jg, Wu Fb, Xi Ym. J Stem Cells Regen Med. 2011 Oct 30;7(2):69-74. Ecollection 2011. Investigation Of Characteristics Of Feather Follicle Stem Cells And Their Regeneration Potential.
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