Anti-NETO1 Antibody

Product Name

Anti-NETO1 Antibody

View all NETO1 Antibodies

SKU/Catalog Number

A08303

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-NETO1 Antibody (Catalog # A08303). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

NETO1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-NETO1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08303)

Host

Rabbit

Contents

NETO1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

NETO1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human NETO1. The immunogen is located within amino acids 350 - 400 of NETO1.

Cross-reactivity

NETO1 antibody is predicted to not cross-react with other NETO protein family members.

Reactive Species

A08303 is reactive to NETO1 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

60.2 kDa

Background of NETO1

Neuropilin and tolloid-like protein 1 (NETO1) is involved in the development and/or maintenance of neuronal circuitry. It is a type I transmembrane protein that is expressed in the brain and retina. NETO1 contains one LDL-receptor class A domain and two CUB domains and is either membrane-bound or secreted. It has three alternatively spliced isoforms, the first two of which are retina-specific and the third of which is found in both retina and brain tissue. Furthermore, as an accessory subunit of the neuronal N-methyl-D-aspartate receptor (NMDAR), it regulates long-term NMDA receptor-dependent synaptic plasticity and cognition, at least in the context of spatial learning and memory.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A08303 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

NETO1 antibody can be used for detection of NETO1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information NETO1

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