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Product Info Summary
| SKU: | A08303-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-NETO1 Antibody Picoband®
SKU/Catalog Number
A08303-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-NETO1 Antibody Picoband® catalog # A08303-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NETO1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08303-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human NETO1 recombinant protein (Position: R5-R514). Human NETO1 shares 95.7% amino acid (aa) sequence identity with mouse NETO1.
Reactive Species
A08303-1 is reactive to NETO1 in Human, Mouse, Rat
Observed Molecular Weight
70 kDa
Calculated molecular weight
60.2 kDa
Background of NETO1
Neuropilin (NRP) and tolloid (TLL)-like 1 is a protein that in humans is encoded by the NETO1 gene. This gene encodes a transmembrane protein containing two extracellular CUB domains followed by a low-density lipoprotein class A (LDLa) domain. This protein is thought to play a critical role in spatial learning and memory by regulating the function of synaptic N-methyl-D-aspartic acid receptor complexes in the hippocampus. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08303-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human RT4 whole cell, human U20S whole cell, human PC-3 whole cell, human SIHA whole cell, rat C6 whole cell, rat NRK whole cell, mouse RM-1 whole cell, mouse SP2/0 whole cell
Validation Images & Assay Conditions
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Western blot analysis of NETO1 using anti-NETO1 antibody (A08303-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human U20S whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human SIHA whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: rat NRK whole cell lysates,
Lane 7: mouse RM-1 whole cell lysates,
Lane 8: mouse SP2/0 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NETO1 antigen affinity purified polyclonal antibody (Catalog # A08303-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NETO1 at approximately 70 kDa. The expected band size for NETO1 is at 60 kDa.
Specific Publications For Anti-NETO1 Antibody Picoband® (A08303-1)
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