Anti-NETO1 Antibody Picoband™

Product Name

Anti-NETO1 Antibody Picoband™

View all NETO1 Antibodies

SKU/Catalog Number

A08303-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-NETO1 Antibody Picoband™ catalog # A08303-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-NETO1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08303-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Immunogen

E.coli-derived human NETO1 recombinant protein (Position: R5-R514). Human NETO1 shares 95.7% amino acid (aa) sequence identity with mouse NETO1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A08303-1 is reactive to NETO1 in Human, Mouse, Rat

Applications

A08303-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

70 kDa

Calculated molecular weight

60.191kDa

Background of NETO1

Neuropilin (NRP) and tolloid (TLL)-like 1 is a protein that in humans is encoded by the NETO1 gene. This gene encodes a transmembrane protein containing two extracellular CUB domains followed by a low-density lipoprotein class A (LDLa) domain. This protein is thought to play a critical role in spatial learning and memory by regulating the function of synaptic N-methyl-D-aspartic acid receptor complexes in the hippocampus. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of NETO1 using anti-NETO1 antibody (A08303-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human U20S whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human SIHA whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: rat NRK whole cell lysates,
Lane 7: mouse RM-1 whole cell lysates,
Lane 8: mouse SP2/0 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NETO1 antigen affinity purified polyclonal antibody (Catalog # A08303-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NETO1 at approximately 70 kDa. The expected band size for NETO1 is at 60 kDa.

Gene/Protein Information For NETO1 (Source: Uniprot.org, NCBI)

Alternative Names

BCTL1; Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor classA domains protein 1; BTCL1; BTCL1BCTL1neuropilin and tolloid-like protein 1; FLJ41325; NETO1; neuropilin (NRP) and tolloid (TLL)-like 1 NETO1 BCTL1, BTCL1 neuropilin and tolloid like 1 neuropilin and tolloid-like protein 1|brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1|neuropilin (NRP) and tolloid (TLL)-like 1

For more info on NETO1, check out the NETO1 Infographic

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In this infographic, you will see the following information for NETO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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