Anti-NETO2 Antibody Picoband™

Product Name

Anti-NETO2 Antibody Picoband™

View all Neto2 Antibodies

SKU/Catalog Number

A09555-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-NETO2 Antibody Picoband™ catalog # A09555-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-NETO2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09555-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Immunogen

E.coli-derived human NETO2 recombinant protein (Position: I23-F525). Human NETO2 shares 97.8% and 97.4% amino acid (aa) sequence identity with mouse and rat NETO2, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A09555-1 is reactive to Neto2 in Human, Mouse, Rat

Applications

A09555-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

65 kDa

Calculated molecular weight

59.368kDa

Background of Neto2

This gene encodes a predicted transmembrane protein containing two extracellular CUB domains followed by a low-density lipoprotein class A (LDLa) domain. A similar gene in rats encodes a protein that modulates glutamate signaling in the brain by regulating kainate receptor function. Expression of this gene may be a biomarker for proliferating infantile hemangiomas. A pseudogene of this gene is located on the long arm of chromosome 8. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of NETO2 using anti-NETO2 antibody (A09555-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SIHA whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NETO2 antigen affinity purified polyclonal antibody (Catalog # A09555-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NETO2 at approximately 65 kDa. The expected band size for NETO2 is at 59 kDa.

Gene/Protein Information For Neto2 (Source: Uniprot.org, NCBI)

Alternative Names

Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor classA domains protein 2; BTCL2; FLJ10430; FLJ90456; NEOT2; NEOT2FLJ14724; NETO2; neuropilin (NRP) and tolloid (TLL)-like 2; neuropilin and tolloid-like protein 2 Neto2|5530601C23Rik, AI854825, BTCL2|neuropilin (NRP) and tolloid (TLL)-like 2|neuropilin and tolloid-like protein 2|brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 2

For more info on Neto2, check out the Neto2 Infographic

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In this infographic, you will see the following information for Neto2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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