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Product Info Summary
| SKU: | PA1662 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Netrin 1/NTN1 Antibody
SKU/Catalog Number
PA1662
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Netrin 1/NTN1 Antibody catalog # PA1662. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Netrin 1/NTN1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1662)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Netrin 1, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1662 is reactive to NTN1 in Human, Mouse, Rat
Applications
PA1662 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
8 kDa
Calculated molecular weight
67.748kDa
Background of Netrin-1
Netrin-1 (NTN1), also known as NTN1L, is a diffusible protein made by floor plate cells. Netrin-1 is found in the floor plate and neuroepithelial cells of the ventral region of the spinal cord, as well as other locations in the nervous system including the somatic mesoderm, pancreas and cardiac muscle. The human NTN1 gene is mapped to chromosome 17p13-p12 by FISH. Netrin-1 can attract spinal commissural axons and repel trochlear axons in vitro. And netrin-1 expression confers a selective advantage for tumor cell survival. The binding of netrin-1 to DCC appears to depend on the presence of a coreceptor or accessory proteins. Adenosine A2b receptor is actually a netrin-1 receptor and induces cAMP accumulation on binding netrin-1, and that netrin-1-dependent outgrowth of dorsal spinal cord axons directly involves A2b. Netrin-1 can promote intestinal tumor development, probably by regulating cell survival. Thus, a netrin-1 receptor or receptors function as conditional tumor suppressors. Expression of Unc5b triggered endothelial cell repulsion in response to netrin-1 in vitro, whereas a truncated Unc5b lacking the intracellular signaling domain failed to induce repulsion.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Validation Images & Assay Conditions
Click image to see more details
Anti-Netrin 1 antibody, PA1662, Western blotting
Lane 1: U87 Cell Lysate
Lane 2: COLO320 Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For NTN1 (Source: Uniprot.org, NCBI)
Gene Name
NTN1
Full Name
Netrin-1
Weight
67.748kDa
Alternative Names
netrin 1; Netrin1; Netrin-1; NTN1; NTN1Lnetrin 1, mouse, homolog of NTN1 MRMV4L, NTN1 netrin 1 netrin-1|epididymis tissue protein Li 131P|netrin 1, mouse, homolog of
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NTN1, check out the NTN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NTN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Netrin 1/NTN1 Antibody (PA1662)
Hello CJ!
PA1662 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhong S, Zou L, Zhao Y, Hu B, Xie H. J Huazhong Univ Sci Technolog Med Sci. 2010 Aug;30(4):500-4. Doi: 10.1007/S11596-010-0457-X. Epub 2010 Aug 17. Effect Of Different Concentrations Of Neogenin On Proliferation, Apoptosis And Related Proliferativ...
Qian-Hua W, Shao-Ping Z, Jian-Wen Z, Yun Y, Li Z. Mol Cell Biochem. 2011 Apr;350(1-2):81-7. Doi: 10.1007/S11010-010-0684-2. Epub 2010 Dec 31. Reduced Expression Of Netrin-1 Is Associated With Fetal Growth Restriction.
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Netrin 1/NTN1 Antibody
Question
I have a question about product PA1662, anti-Netrin 1/NTN1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1662 anti-Netrin 1/NTN1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-11
Question
Does PA1662 anti-Netrin 1/NTN1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-06-27
Answer
It shows on the product datasheet, PA1662 anti-Netrin 1/NTN1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-06-27
Question
We are currently using anti-Netrin 1/NTN1 antibody PA1662 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2017-10-24
Answer
The anti-Netrin 1/NTN1 antibody (PA1662) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-24
