Anti-Neurofilament NF-M Antibody

Boster Bio Anti-Neurofilament NF-M Antibody catalog # M06821-3. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Chicken, Equine, Pig.

Product Info Summary

SKU: M06821-3
Size: 100ul
Reactive Species: Bovine, Chicken, Equine, Pig
Host: Chicken
Application: IF, IHC, WB

Product Name

Anti-Neurofilament NF-M Antibody

See all NF-M products

SKU/Catalog Number

M06821-3

Size

100ul

Description

Boster Bio Anti-Neurofilament NF-M Antibody catalog # M06821-3. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Chicken, Equine, Pig.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Neurofilament NF-M Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06821-3)

Host

Chicken

Contents

Purified IgY preparation at 20-30 mg/mL with 5mM NaN3

Clonality

Polyclonal

Immunogen

Recombinant construct containing the C-terminus of the human sequence (amino acids 708-877) expressed in and purified from E. coli.

Reactive Species

M06821-3 is reactive to NEFM in Bovine, Chicken, Equine, Pig

Applications

M06821-3 is guaranteed for IF, IHC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Use 1:10,000 for WB, 1:1,000 for ICC

Validation Images & Assay Conditions

Gene/Protein Information For NEFM (Source: Uniprot.Org, NCBI)

Uniprot ID

P07197

Gene ID

4741

Gene Name

NEFM

Full Name

Neurofilament medium polypeptide

Weight

102.472kDa

Superfamily

intermediate filament family

Alternative Names

NEF3; NEF3neurofilament medium polypeptide; NEFM; Neurofilament 3; neurofilament, medium polypeptide 150kDa; neurofilament, medium polypeptide; neurofilament-3 (150 kD medium); NFM; NF-M; NF-M160 kDa neurofilament protein; NFMNeurofilament triplet M protein NEFM NEF3, NF-M, NFM neurofilament medium neurofilament medium polypeptide|160 kDa neurofilament protein|neurofilament 3|neurofilament triplet M protein|neurofilament, medium polypeptide 150kDa|neurofilament-3 (150 kD medium)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on NEFM, check out the NEFM Infographic

NEFM infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for NEFM: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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4 Customer Q&As for Anti-Neurofilament NF-M Antibody

Question

I have a question about product M06821-3, anti-Neurofilament NF-M antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-01-06

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M06821-3 anti-Neurofilament NF-M antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-01-06

Question

We are currently using anti-Neurofilament NF-M antibody M06821-3 for pig tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says bovine, chicken, equine, pig. Is it true that the antibody can work on feline tissues as well?

A. Rodriguez

Verified customer

Asked: 2018-03-09

Answer

The anti-Neurofilament NF-M antibody (M06821-3) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-03-09

Question

Is this M06821-3 anti-Neurofilament NF-M antibody reactive to the isotypes of NEFM?

Verified Customer

Verified customer

Asked: 2017-10-05

Answer

The immunogen of M06821-3 anti-Neurofilament NF-M antibody is Recombinant construct containing the C-terminus of the human sequence (amino acids 708-877) expressed in and purified from E. coli.. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-10-05

Question

Would anti-Neurofilament NF-M antibody M06821-3 work for IF with retina?

T. Johnson

Verified customer

Asked: 2017-07-05

Answer

According to the expression profile of retina, NEFM is highly expressed in retina. So, it is likely that anti-Neurofilament NF-M antibody M06821-3 will work for IF with retina.

Boster Scientific Support

Answered: 2017-07-05

$299

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Ships directly to USA and Canada. For other regions, visit our distributors page

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In stock
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M06821-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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