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Product Info Summary
| SKU: | A01290-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-Neuropeptide S/NPS Antibody
SKU/Catalog Number
A01290-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Neuropeptide S/NPS Antibody Picoband® catalog # A01290-1. Tested in IHC applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Neuropeptide S/NPS Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01290-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Neuropeptide S, which shares 80% amino acid (aa) sequence identity with both mouse and rat Neuropeptide S.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01290-1 is reactive to NPS in Mouse, Rat
Calculated molecular weight
10.1 kDa
Background of NPS
Neuropeptide S (NPS) is a neuropeptide found in human and mammalian brain, mainly produced by neurons in the amygdala and between Barrington's nucleus and the locus coeruleus, although NPS-responsive neurons extend projections into many other brain areas. NPS binds specifically to a G protein-coupled receptor, NPSR. Animal studies show that NPS suppresses anxiety and appetite, induces wakefulness and hyperactivity, including hyper-sexuality, and plays a significant role in the extinction of conditioned fear.It has also been shown to significantly enhance dopamine activity in the mesolimbic pathway, and inhibits motility and increases permeability in neurocrine fashion acting through NO in the myenteric plexus in rats and humans.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01290-1 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat
Positive Control
IHC: rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
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IHC analysis of Neuropeptide S using anti-Neuropeptide S antibody (A01290-1).
Neuropeptide S was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Neuropeptide S Antibody (A01290-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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IHC analysis of Neuropeptide S using anti-Neuropeptide S antibody (A01290-1).
Neuropeptide S was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Neuropeptide S Antibody (A01290-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Neuropeptide S/NPS Antibody (A01290-1)
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5 Customer Q&As for Anti-Neuropeptide S/NPS Antibody
Question
Would A01290-1 anti-Neuropeptide S/NPS antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
You can see on the product datasheet, A01290-1 anti-Neuropeptide S/NPS antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-05-07
Question
Do you have a BSA free version of anti-Neuropeptide S/NPS antibody A01290-1 available?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Neuropeptide S/NPS antibody A01290-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-28
Question
Will anti-Neuropeptide S/NPS antibody A01290-1 work on zebrafish WB with placenta?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
Our lab technicians have not validated anti-Neuropeptide S/NPS antibody A01290-1 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Neuropeptide S/NPS antibody A01290-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish placenta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-22
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-Neuropeptide S/NPS antibody A01290-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-05-01
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-01
Question
We are currently using anti-Neuropeptide S/NPS antibody A01290-1 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on canine tissues as well?
C. Bhatt
Verified customer
Asked: 2017-10-13
Answer
The anti-Neuropeptide S/NPS antibody (A01290-1) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-13
