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- Table of Contents
NRP1 antibody (M01324) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 7 Publications
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IHC analysis of NRP1 using anti-NRP1 antibody (M01324).
NRP1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-NRP1 Antibody (M01324) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
NRP1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-NRP1 Antibody (M01324) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Neuropilin 1 NRP1 Rabbit Monoclonal Antibody
NRP1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
NRP1 antibody was positively detected by WB in:
human PC-3 whole cell, human A549 whole cell, human U251 whole cell, rat heart tissue, rat brain tissue, mouse heart tissue, mouse brain tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
NRP1 antibody was positively detected by IHC in:
human liver cancer tissue, human stomach cancer tissue, rat brain tissue, rat brain tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
IP
FC
Human
Tested
Tested
Recommend
Recommend
Recommend
Recommend
Rat
Tested
Tested
Recommend
Recommend
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-Neuropilin 1 NRP1 Rabbit Monoclonal Antibody
View all NRP1 Antibodies
View all NRP1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BGO-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Neuropilin 1
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
120 kDa
Calculated Molecular Weight
103.1 kDa
Cite This Product
Anti-Neuropilin 1 NRP1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01324)
Protocols
Western Blot protocol for NRP1 antibody
Immunohistochemistry (IHC) protocol for NRP1 antibody
Publications
Application:
Species:
Interaction between CD82 and integrin αVβ3 selectively regulates collective movement of tumor cells via endolysosomal trafficking
Published: 2026-06-22
Journal: CELLULAR AND MOLECULAR LIFE SCIENCES
Application: IF
Impact Factor: 6.50
Species: Human
PMID: 42332332
Developmental exposure to methyl ester sulfonate induces autism-like behavioral deficits in mice by dysregulation of the Notch/Hes signaling pathway disrupting neuronal differentiation
Published: 2025-08-19
Journal: ARCHIVES OF TOXICOLOGY
Application: WB
Impact Factor: 6.90
Species: Mouse
PMID: 40828199
Sex-specific molecular hallmarks point to increased atherogenesis susceptibility in male senescence-accelerated mice
Published: 2025-03-04
Journal: LIFE SCIENCES
Application: WB
Impact Factor: 5.10
Species: Mouse
PMID: 40049367
A dual receptors-targeting and size-switchable “cluster bomb” co-loading chemotherapeutic and transient receptor potential ankyrin 1 (TRPA-1) inhibitor for treatment of triple negative breast cancer
Published: 2020-02-05
Journal: JOURNAL OF CONTROLLED RELEASE
Application: WB
Impact Factor: 7.73
Species: Mouse
PMID: 32035191
Shear Stress Promotes Arterial Endothelium-Oriented Differentiation of Mouse-Induced Pluripotent Stem Cells
Published: 2019-11-15
Journal: Stem Cells International
Application: WB
Impact Factor: 3.90
Species: Mouse
PMID: 31827524
Dual receptor recognizing liposomes containing paclitaxel and hydroxychloroquine for primary and metastatic melanoma treatment via autophagy-dependent and independent pathways
Published: 2018-08-09
Journal: JOURNAL OF CONTROLLED RELEASE
Application: WB
Impact Factor: 7.88
Species: Mouse,Human
PMID: 30099017
Dual Receptor Recognizing Cell Penetrating Peptide for Selective Targeting, Efficient Intratumoral Diffusion and Synthesized Anti-Glioma Therapy
Published: 2016-01-01
Journal: Theranostics
Application: WB
Impact Factor: 8.85
Species: Human,Mouse
PMID: 26877777
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We promise Anti-Neuropilin 1 NRP1 Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M01324
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 18, 2026