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Product Info Summary
| SKU: | M03534 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, IHC |
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Product info
Product Name
Anti-Next to BRCA1 gene 1 protein NBR1 Monoclonal Antibody
SKU/Catalog Number
M03534
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Next to BRCA1 gene 1 protein NBR1 Monoclonal Antibody catalog # M03534. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Next to BRCA1 gene 1 protein NBR1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03534)
Host
Mouse
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
6A12-C8
Isotype
IgG
Immunogen
Recombinant Protein of NBR1
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M03534 is reactive to NBR1 in Human, Mouse, Rat
Calculated molecular weight
107.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03534 is guaranteed for IF, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF 1:50-200
IHC 1:50-300
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, NBR1 Mouse Monoclonal Antibody (7C3) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
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Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, NBR1 Mouse Monoclonal Antibody (7C3) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
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Immunofluorescence analysis of rat-spleen tissue. 1, NBR1 Mouse Monoclonal Antibody (7C3) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Specific Publications For Anti-Next to BRCA1 gene 1 protein NBR1 Monoclonal Antibody (M03534)
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