Anti-Next to BRCA1 gene 1 protein NBR1 Monoclonal Antibody

Product Name

Anti-Next to BRCA1 gene 1 protein NBR1 Monoclonal Antibody

View all NBR1 Antibodies

SKU/Catalog Number

M03534

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Next to BRCA1 gene 1 protein NBR1 Monoclonal Antibody catalog # M03534. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Next to BRCA1 gene 1 protein NBR1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03534)

Host

Mouse

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Monoclonal

Clone Number

6A12-C8

Isotype

IgG

Immunogen

Recombinant peptide derived from NBR1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

M03534 is reactive to NBR1 in Human, Mouse, Rat

Applications

M03534 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

107.413kDa

Background of NBR1

Anti Mesothelin Antibody recognizes Mesothelin that is a glycosyl-phosphatidylinositol–anchored glycoprotein present on the cell surface of various human solid tumors. The mesothelin (MSLN) gene encodes a 71-kDa precursor protein that is processed to a 40-kDa glycosylphosphatidylinositol–anchored protein that composes the mature portion and an NH2 terminal 31-kDa fragment called megakaryocyte-potentiating factor that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues, such as mesothelium, but aberrantly over expressed in mesotheliomas, ovarian, and pancreatic cancers. The biological functions of mesothelin remain elusive. A recent study showed that mesothelin binds to MUC16/CA125, and that this interaction mediates cell adhesion, suggesting that there may be an important role for MUC16/CA125 and mesothelin in the metastatic spread of ovarian cancer.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-2000
ELISA 1:10000-20000

Validation Images & Assay Conditions

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Figure 2. Immunohistochemistry validation of NBR1 using Anti-Next to BRCA1 gene 1 protein NBR1 Monoclonal Antibody (M03534).

Immunohistochemical analysis of human-liver tissue. Anti-NBR1 at 1:200 (4°C

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Figure 3. Immunohistochemistry validation of NBR1 using Anti-Next to BRCA1 gene 1 protein NBR1 Monoclonal Antibody (M03534).

Immunohistochemical analysis of mouse heart tissue. Anti-NBR1 at 1:200 (4°C

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Figure 4. Immunohistochemistry validation of NBR1 using Anti-Next to BRCA1 gene 1 protein NBR1 Monoclonal Antibody (M03534).

Immunohistochemical analysis of mouse lung tissue. Anti-NBR1 at 1:200 (4°C

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Figure 5. Immunohistochemistry validation of NBR1 using Anti-Next to BRCA1 gene 1 protein NBR1 Monoclonal Antibody (M03534).

Immunohistochemical analysis of rat lung tissue. Anti-NBR1 at 1:200 (4°C

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Figure 6. Immunohistochemistry validation of NBR1 using Anti-Next to BRCA1 gene 1 protein NBR1 Monoclonal Antibody (M03534).

Immunohistochemical analysis of paraffin-embedded Human Skeletal Muscle Tissue using NBR1 Mouse monoclonal antibody diluted at 1:200.
For more protocol information of IHC

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Figure 1. Western blotting validation for Anti-Next to BRCA1 gene 1 protein NBR1 Monoclonal Antibody M03534

Western blot (WB) analysis of Anti-NBR1 antibody.
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For NBR1 (Source: Uniprot.org, NCBI)

Alternative Names

Cell migration-inducing gene 19 protein; FLJ98272; KIAA0049CA125; M17S2FLJ55359,1A1-3B; Membrane component chromosome 17 surface marker 2,1A13B; membrane component, chromosome 17, surface marker 2 (ovarian carcinoma antigenCA125); migration-inducing protein 19; Neighbor of BRCA1 gene 1 protein; neighbor of BRCA1 gene 1; next to BRCA1 gene 1 protein; Protein 1A1-3B NBR1 1A1-3B, IAI3B, M17S2, MIG19 NBR1 autophagy cargo receptor next to BRCA1 gene 1 protein|B-box protein|cell migration-inducing gene 19 protein|membrane component, chromosome 17, surface marker 2 (ovarian carcinoma antigen CA125)|migration-inducing protein 19|neighbor of BRCA1 gene 1

For more info on NBR1, check out the NBR1 Infographic

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In this infographic, you will see the following information for NBR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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